Identify pathogens which causing food poisoning, analysis of genetic relationships among strains and provide the basis for the diagnosis of food poisoning clear.MethodsUsing quantitative PCR rapid screening, GB 4789.4-2010 method to separate and identify the pathogens. ResultsFrom the 16 cake samples detected 8 Salmonella enteritidis, 32 stool samples of patients detected 17 Salmonella enteritidis, PCR nucleic acid positive and the GB 4789.4-2010 method were isolated with the same strains. PFGE banding showed that patients had the same pattern of Salmonella enteritidis with which were detected in cake, and they had high homology.ConclusionThis food poisoning from Salmonella enteritidis contamination by the cake. PCR method with the GB 4789.4-2010 method helps to quickly lock joint detection of food poisoning bacteria. PFGE detected Salmonella enteritidis of the cake and patients were sourced from the same clone. It demonstrate the relevance of food pathogens from genes level.