To establish a method of high performance liquid chromatography (HPLC) for the determination (ADA) of azodicarbonamide in flour. MethodsSamples were extracted with acetone, then centrifuged and concentrated, the residue was dissolved with water. The injection was separated by cyano column and detected by photo-diode array. ResultsThe method showed a good linearity in the range of 2-40 μg/ml (r=0.999 8). The recoveries of ADA from flour spiked at three levels were in the range of 74%-118% with the relative standard deviations less than 5%. The limit of quantification (LOQ) was 3 mg/kg.ConclusionThis method could be used for screening and quantification of ADA in flour for its strong specificity and simple preparation.