Research Center of Physics and Chemistry Analysis,Key Laboratory of Analytical Science and Technology of Hebei Province,Hebei University,Hebei Baoding 071002,China 在期刊界中查找 在百度中查找 在本站中查找
Research Center of Physics and Chemistry Analysis,Key Laboratory of Analytical Science and Technology of Hebei Province,Hebei University,Hebei Baoding 071002,China 在期刊界中查找 在百度中查找 在本站中查找
Research Center of Physics and Chemistry Analysis,Key Laboratory of Analytical Science and Technology of Hebei Province,Hebei University,Hebei Baoding 071002,China 在期刊界中查找 在百度中查找 在本站中查找
The aim was to establish a method for the simultaneous determination of 2kinds of cholesterol oxidation products (COPs:7-ketocholesterol,cholesta-4,6-dien-3-one) by HPLC.MethodsThe mixture of samples and Florisil packing were grinded, then packed to a syringe column. The extraction and purification of the samples were carried out with one-step using acetonitrile as eluting reagent. With anhydrous ethanol as mobile phase, the two components were determined by liquid chromatography under 240-285nm. ResultsThere was a good linearity in the range of 0.25-100μg/ml and the correlation coefficients were greater than 0.9993. The detection limits for 2COPs were 0.015and 0.045μg/g, respectively. Under the optimal conditions, the recovery rates of the two target analytes were between 74.1%-97.0% and the relative standard deviations (RSDs) were no more than 5.57%.ConclusionThis method is suitable for the determination of cholesterol oxides in chicken liver.