In order to investigate the effects of bisphenol A (BPA) on testicle tissue of mice from toxicologic pathological perspective, and providd morphological evidences of BPA toxicity.Methods108SPF CD-1male mice were randomly allocated into four groups with BPA concentration of 0,0, 300and 600mg/kg BW. All BPA groups were administrated orally with different doses for 8weeks continuously. Then testicles were taken and fixed to perform pathological observation and analysis. ResultsAfter 8weeks of BPA administration, body weight and testicle index of BPA groups were decreased compared to control group. Degeneration and necrosis were observed in sustentacular cell of seminiferous tubule, and spermatozoon number decreased. Karyopyknosis and maldevelopment were occurred at acrosome vesicle and cap of spermatogenic cells observed by TEM. Immunohistochemistry test showed that NF-κB and Caspase-3expression were up-regulated (P＜0.01or P＜0.05) in BPA groups compared to control group. TUNEL test indicated that the number of apoptosis positive cells was higher in BPA groups than control group (P＜0.01).ConclusionDifferent doses of BPA could lead to testicle tissue damage at different level, and induce cell apoptosis of testicle.