A method for determination of 49pesticide residues in mushroom and ginger by SPE-GC/MS was established, and 74mushroom and 22ginger samples were detected.MethodsThe pesticide residues in fresh mushroom and ginger were extracted with acetonitrile, and dry mushroom was extracted with acetone. The extracts were purified by SPE after being concentrated, and DB-1701capillary column was used for separation. ResultsThere was a good linearity between the peak area and the concentration of 49pesticide residues within 0.25μg/ml-2μg/ml, the correlation coefficients were 0.9890-0.9997, the recoveries of spiked sample at 8and 80μg/kg to fresh mushroom and ginger were ranged from 66.5%-143.8%, RSDs were ranged from 2.2%-10.8%, and the detection limits were ranged from 0.1-10.0μg/kg. ConclusionThe method was simple, rapid and reliable. It could be applied to determine pesticide residues in fresh mushroom and ginger.