Phytosterols is a kind of steroidal compoundwith perhydrocyclopentanophenanthrene as the main structure. Phytosterols are natural components of oil seeds, legumes, and grains. It has been reported that phytosterols could prevent and cure prostate glanddisease and act as immune system modulators,and they also have antiinflammation and anticancer properties.Presently, dietary application of phytosterols and phytosterolesters keeps increasing in many food types.In this paper, the application and safety issue of novel food phytosterols is discussed.