To establish a method for the determination of chlorpromazine in pork by gas chromatography-mass spectrometry.Methods The sample was homogenized and extracted by ethyl acetate, degreased by hexane and acetonitrile, purified by solid phase extraction, and eluted by 5% ammoniated methanol. Ion fragments of 86,3, 272and 318were selected for qualitative and quantitative analysis by gas chromatography-mass spectrometry.Results Good linearities were shown in 0.051-0.25μg/ml with the relative coefficients of 0.9992. The limit of detection was 2.0μg/kg. The average recoverys were from 87.1% to 97.3% and the relative standard derivation was 3.15%.Conclusion It could be applied to determine the chlorpromazine in pork.