To establish a method for simultaneous determination of 15PAHs in plant oil by high performance liquid chromatography with fluorescence detection.Methods The sample was dissolved in n-hexaneand, and cleaned up with neutral alumina SPE cartridges．The separation of 15PAHs was carried out by waters-PAHs column (4.6mm×250mm, 5μm) with a gradient elution using acetonitrile-water as mobile phase at a flow rate of 1.5ml/min, the column temperature was 30℃, and the injection volume was 10μl. Detection was carried out by a fluorescence detector with external standard. Results The limit of detection (LOD) was in the range of 0.15～2.0μg/kg with average recovery ranging from 78.0% to 115.2%. The relative standard deviation was in the range of 0.2%～2.0%.Conclusion The method had the advantages of simple pretreatment, high sensitivity and reproducibility, which could be applied to the determination of the 15PAHs in plant oil.