Anoval method of nano-immunomagnetic separation (Nano-IMS) plus loop-mediated isothermal amplification (LAMP) was established for the rapid detection of Vibrio parahemolyticus.MethodsThe Nano-Immunomagnetic Beads (Nano-IMB) were created by using the monoclonal antibody of V. parahemolyticus and named Nano-IMB-Vp, which was highly specific to V. parahemolyticus. The method of Nano-IMS-LAMP was established for the rapid detection of V. parahemolyticus from seefoods. ResultsThe capture ratio of Nano-IMB-Vp was reached 74% at the level of 103cfu/ml. In pure culture, the sensitivity of Nano-IMS-LAMP was reached 140cfu/ml culture medium. The specific of this LAMP method was tested by using 134targets and 74non-targets bacteria. The results showed that the LAMP method was highly specific to V. parahemolyticus. Nocross-reaction was founded. The detection limit of Nano-IMS-LAMP reached 2cfu/25g in artficial samples when the period of time for enrichment was shorted to 8h.ConclusionThe method of Nano-IMS-LAMP could shorten the period of time for enrichment effectively, which can apply to the rapid detection of V. parahemolyticus.