objective To understand the presence, contamination and cross contamination of foodborne bacterial pathogens in Ningbo city, provide basis for foodborne disease control, and trace the source of foodborne disease.MethodsStrains were detected directly or after enrichment with biochemistry and API method, and subtyped with serum agglutination method. Antibiotic resistance and relative genes were detected with K-B method and PCR method respectively. Results 2 331(7 species and 12 types)strains were detected from 6 812 food samples and the detection rate is 34.22%(2 331/6 812).The prevalent pathogens were Vibrio parahaemolyticus, and the detection rate was significantly different from the other types(P<0.005).Vibrio parahaemolyticus could be classified into 10 sero-groups, and O6 and O5 were proved as the prevalent sero-groups. Most of the pathogens were sensitive to antibiotics. Three strains of Aeromonas were found multi-resistant with aacc resistance gene. Conclusion Various distribution was proved in foodborne bacteria in Ningbo. Contamination of foodborne pathogens was a major factor of foodborne diseases. Vibrio parahaemolyticus was the prevalent pathogenic bacteria. Most of the pathogens were sensitive to antibiotics. Bacteria with aacc resistance gene were found, which should raise concerns to control the spread of the resistant strains through rational administration of antibiotics and resistance surveillance.