To study the pulse-field gel electrophoresis(PFGE)type of Salmonella strains isolated from poultry slaughterhouses in four provinces. Methods DNA collected from 167 Salmonella strains were digested by XbaI according to the standard PFGE protocol of US CDC. The PFGE patterns were analyzed with BioNumerics software. Results167 Salmonella strains were clustered into 18 groups with 75 different patterns, and the same serotype had the similar PFGE pulse types which could be classified to one group. 65 S.Indiana were clustered into 38 patterns. 54 S.Enteritidis were clustered into 16 patterns. 16 S.Albany were clustered into 1 pattern. Conclusion The distribution of pulse types of Salmonella isolates was different between regions, but some pulse types were cross-distributed. Cross-contamination was serious in slaughterhouses. Monitoring and intervention of critical control point during processing should be strengthened in order to decrease salmonella contamination in poultry products, and that was the basic measure of source control.