A method was developed for the simultaneous determination of 13quinolone (QNs) antibiobtics in Swine muscle by ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS).Methods The analytes were extracted from the sample using phosphate solution with ultrasonic bath and purified by anion exchange solid-phase extraction(SPE)cartridge. The residue was dried under nitrogen and redissolved for UPLC-MS/MS. The interference of matrix was reduced by the matrix-matched calibration standards curve. Results The linearity arrange was from 5μg/kg to 100μg/kg for 13QNs with correlation coefficients from 0.9910-0.9992. The limit of quantification was 0.255-3.322μg/kg. The recoveries were 69.18%-104.45% with relative standard deviations of 0.21%-10.98%. Conclusion The analytical method is simple, accurate, sensitive and suitable for the determination of 13quinolone (QNs) antibiobtics in swine muscle.