To develop a new method for quantitative determination of 65herbicides belonging to different chemical families in vegetables by gas chromatography-mass spectrometry(GC-MS)．MethodsSamples were ultrasonically extracted with acetonitrile and the extracts were cleanuped with Carb/NH₂ cartridge before GC-MS analysis．Matrix-matched calibration was used for quantification．ResultsThe linearity was satisfying within the range of 10～1000μg/L,and the coefficients of determination(r)were above 0.998．The limits of detection(LODs)and the limits of quantification(LOQs)were 0.02～2.2μg/kg and 0.1～7.4μg/kg, respectively. The recoveries for spiked samples at the levels of 5,10and 20μg/kg ranged from 60.9% to 117.9%, except for EPTC and Etobenzanid in the range of 41.1%～59.4%．The relative standard deviations(RSD)were below 14.0%． Trifluralin was detected in 1out of 9vegetable samples collected from local market,with the concentration of 2.0μg/kg．Conclusion The proposed method was rapid,simple and sensitive for the analysis of 65herbicides in vegetables．