To predict the allergenic potential of recombinant human α-lactalbumin (rhLA) expressed in the milk of transgenic cloned cattle using bioinformatics analysis.Methods The Allergen Online Database, Allergen Database for Food Safety and Structural Database of Allergenic Proteins were used to determine the similarity of amino acid sequences and structures between the expressed protein and known allergens. Results There was a high level of similarity between rhLA and some known allergens, including bevine alpha lactalbumin, bovine lactose synthas B proteina (Bos d 4) and chicken lysozyme C (1,4-beta-N-acetylmuramidase C, Gal d 4, Gal d IV).ConclusionRhLA has possible allergenic potential.