A method of high performance liquid chromatography was established for simultaneous determination of salidroside and adenosine in healh food.Methods Salidroside and adenosine in samples were extracted ultrasonically with 50%methanol as the solvent and separated on a shim-pack CLS-ODS column(150mm×4.6mm,5μm)；the mobile phase consisted of methanol+20mmol/L Potassium dihydrogen phosphate(15+85,V/V). The flow rate was 1.0ml/min; the injection volume was 10μl; the UV detection wavelength of salidroside and adenosine ware 275and 260nm. Results The standard curve of salidroside was linear over the range in 10.0～200μg/ml(r=0.9995).The recovery was 92.5%～99.5%,RSD 2.8%～4.7% (n=6);The standard curve of adenosine was linear over the range in 2.50～50.0μg/ml(r=0.9995).The recovery was 90.0%～94.0%,RSD 2.1%～5.2%(n=6). The detection limits of salidroside and adenosine were 0.01and 0.005μg/g.Conclusion The method was simple, sensitive and accurate, it could be used for simultaneous determination of salidroside and adenosine in health food.