Preliminary assessment of recombinant human lactoferrin for allergenic potential
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摘要:
初步评价牛乳腺生物反应器表达的重组人乳铁蛋白(rhLf)的致敏性。方法通过生物信息学分析和消化稳定性试验了解rhLf致敏的可能性。结果生物信息学分析显示rhLf与已知致敏原乳转铁蛋白和卵转铁蛋白有序列同源性,与Asp f 2、Ole e 10和Ole e 9存在结构相似性。消化稳定性试验显示rhLf在胃液中易被消化成小片段,在肠液中不易被消化。结论rhLf具有一定的潜在致敏可能性。
Abstract:
To evaluate the allergenic potential of recombinant human lactoferrin (rhLf) expressed by genetically modified cow in milk.Methods Bioinformatics searches and digestibility assay were conducted to investigate the allergenic potential of rhLf. [WT5"HZ]Results Bioinformatics searches showed that there was high-level similarity between rhLf and some known allergens, such as lactotransferrin and ovotransferrin in amino acid sequence, and Asp f 2, Ole e 10and Ole e 9in structure. Digestibility assay showed that rhLf broke down into small fragment rapidly during gastric fluid digestion, while it was resistant to intestinal fluid digestion.ConclusionRhLf has allergenic potential.
