Objective To establish a method for the simultaneous determination of ellagic acid and punicalagins in health food by high performance liquid chromatography(HPLC).Methods Chromatographic separation was carried out by using a Diamonsil C18(Ⅱ) column(4.6 mm×250 mm,5 μm).The mobile phases were acetonitrile and 0.2% H3PO4 aqueous solution with an optimized gradient elution.The detective wavelength was 256 nm.Results The detection limits of α ellagic acid,β ellagic acid and punicalagins were 2.36,2.78 and 2.67 μg/g,respectively.The linear range for α ellagic acid,β ellagic acid and punicalagins were 24-240 μg/ml,56.0-560 μg/ml and 60.0-600 μg/ml,respectively,and all of which showed a correlation coefficient of over 0.999 7.The recoveries of three analytes at two spiked levels were ranged from 97.5%-104% and all of the relative standard deviation(RSD) were less than 2.5%.Conclusion This method was validated to be simple,rapid,accurate and reproducible for the determination of Ellagic Acid and punicalagins in health food.