Objective To study the toxicological safety of Acer truncatum Bunge Oil. Methods Acute toxicity and genotoxicity, including Ames test, micronucleus of born marrow and sperm abnormality in mice, and subchronic toxicity test in rats (90-day feeding test) were used to assess the safety of Acer truncatum Bunge Oil. Results The oral LD₅₀ of Acer truncatum Bunge Oil in mice was more than 54000 mg/kg BW, which belonged to an actual non-toxicity substance. The results of genotoxicity test were all negative. Rats were grown healthy and no abnormal indices were shown after test samples being orally given at the dosage of 10800, 5400 and 2700 mg/kgBW for 90 days. Conclusion Acer truncatum Bunge Oil was nontoxic neither on genotoxicity test nor on subchronic toxicity test under the present condition.