Objective To observe the effect of high-lipid diet during early life on the development and serum cholesterol level of their offspring and the protective effects of phytosterol in mice. Method After pregnancy being confirmed，30 ten-week-old healthy C57BL /6J dams were housed individually and randomly divided into basal diet group，high-lipid diet group and high-lipid diet supplemented with 1. 5% phytosterol diet group according to their body weight. Ten weaning male offspring from each group were fed with basal diet and the blood，brain，kidney，liver samples were collected by the end of three months old. Serum lipids were determined by kits ( enzymatic method) . The expression of HMGCoAR was measured by RT-PCR. Results Serum TC and LDL-C levels of the offspring from high-lipid diet group were significantly higher than those from basal diet group. There was no significantly effect of phytosterol in the mother's diet on cholesterol metabolism of the offspring. Conclusion Maternal high-lipid diet could have some effects on the serum cholesterol level and testis development and increase the risk of cardiovascular diseases of the offspring.