Objective To establish a capillary electrophoresis method for detecting the content of β-lacto globulin in dairy products. Method Samples were handled with buffers; an uncoated neutral capillary column in the diameter of 70 μm and the effective length of 600 mm was used. The samples were detected under the condition of capillary temperature at 40℃; citric acid buffer solution at pH 3.0 ± 0.2, operating voltage at 27 kV, detection wavelength at 214 nm, and injection time for 5 s. Results The average recovery was 75.2%–105.2% and the relative standard deviation (RSD) was 1.09%–3.10% for β-lacto globulin. The detection limit was 0.001mg/ml. Conclusion The method is simple, accurate and sensitive. It is suitable for fast screening and quantitative monitoring β-lacto globulin in dairy products.