Objective To establish a cold saponification-HPLC method for the determination of vitamin A and vitamin E in fortified foods. Method Samples were saponified over night, adjusted to pH 6.0?7.5 with acetic acid and determined by HPLC. Chromatographic conditions: using SUPERIOREX ODS Column (4.6 mm×250 mm, 5μm) with a mobile phase of methanol and water (94?6). Results The linearity ranges were 0.25?3.00μg/ml for vitamin A and 10.0-150.0μg/ml for Vitamin γ-E, δ-E and α-E. The correlation coefficients were 0.9997?0.9999. The qualitative detection limit was 0.40μg/g for vitamin A, 5.0μg/g for vitamin γ-E and δ-E and 8.0μg/g for vitamin α-E. The quantitative detection limits of the method were 1.30μg/g for vitamin A, 16.7μg/g for vitamin γ-E and δ-E, and 26.7μg/g for vitamin α-E. The average recoveries of adding standards were 77.0%?105% and the relative standard deviation (RSD) of the method was less than 5%. Conclusion: The method was simple, fast, accurate and reproducible and could be used to determine the contents of Vitamin A and vitamin E in vitamin fortified foods.