Objective To understand the contamination of ochratoxin (OTA) from beer in Beijing and estimate the exposure of OTA in local residents. Methods OTA in beer samples were detected by IAC-HPLC-FD and were evaluated by referencing to the EU dietary exposure assessment methods. Results OTA was found in 39 of 87 beer samples, with a positive rate of 44.83%. The average concentration of OTA was 0.015ng/ml in the range of 0.010 -0.093ng/ml; the average concentration of positive samples was 0.026ng/ml. The intake of OTA per week from beer in Beijing residents was 0.01-0.50ng/kg. Conclusion The contamination of OTA from beer in Beijing was low in comparing with other countries. The exposure to OTA from beer in local residents was lower than 100ng/kg bw of PTWI proposed by JECFA and lower than 5ng/kg BW of TDI proposed by EU.