Objective To establish a method for molecular typing Pseudomonas cocovenenans subsp. farinofermentans with pulsed-field gel electrophoreses (PFGE), and to analyze the type of 29 isolates with PFGE method. Method The isolates were digested with 5 kinds of restriction endonucleases XbaⅠ, SpeⅠ, NotⅠ, SmaⅠ and AscⅠ, and the PFGE patterns were analyzed by BioNumerics software. Results The bands on PFGE for the fragments digested by restriction endonucleases NotⅠ, SmaⅠ or AscⅠwere too small, and that digested by XbaⅠ were too many to identify clearly. The restriction endonucleases NotⅠ, SmaⅠ, Asc and XbaⅠwere not suitable for typing the pathogen on PFGE. The number of bands on PFGE for the fragments digested by SpeⅠ was moderate and the pattern of molecular weight was clear and well discriminated. Conclusion This method can be used for molecular typing and tracing Pseudomonas cocovenenans subsp. farinofermentans.