Objective To establish a source tracing database for Cronobacter spp. Method Establishing a database, including biotying, antimicrobial susceptibility, pulsed-field gel electrophoresis, ribotyping and 16S rRNA gene sequence, of Cronobacter spp., with a BioNumerics software. Results The source tracing database for Cronobacter spp. can be used to develop systematic research about this pathogen and for comparative analysis of different typing methods. Conclusion Based on the techniques and constantly improved information, the database will be helpful to compare timely and effectively the similarity of isolates and estimate the population variations of Cronobacter spp.. The database will provide scientific information for tracing the source of sporadic cases, outbreaks and for active surveillance of foodborne disease caused by this pathogen.