Objective To investigate the modelling of ovariectomized rats for climacteric syndrome, in order to provide scientific basis for the functional assessment of products by which assist in improving climacteric syndrome. Method Seventy two rats were randomly divided into six groups of 12 rats each. The sham-operated (SH) control group was given water (ig), another five ovariectomized groups were dosed with water(ig)(OVX group), 0.2mg/kg estradiol (ip ) and 50, 100, 200mg/kg soybean isoflavones (ig) respectively. All rats were fed with soy-free chow. Tail temperature and blood E₂, FSH, LH and β-EP of all rats were measured. Results The tail temperature of OVX group was significantly higher than SH group. The tail temperature of estradiol group and isoflavones groups was lower than SH group. Blood estradiol of OVX group were significantly lower than other groups. LH of OVX group was significantly higher than SH group and LH of estradiol group and 200mg/kg soybean isoflavones was lower than SH group. FSH and β-EP of OVX group was significantly higher and lower than other groups respectively. Conclusion Ovariectomized rat model can be used as a simulator for climacteric syndrome, and the signs of climacteric syndrome in this model can be improved by soybean isoflavones.