软烤扇贝加工源头的细菌控制研究
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许 钟 女 研究员 研究方向为水产食品有害微生物 生态与动态学 E2mail: xuzhong@smmail. Cn

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中央级公益性科研院所基本科研业务费专项资金资助项 目(2007M05)


Control of Bacteria in Lightly Baked Scallop from the Management of Raw Materials
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    摘要:

    目的 研究鲜活扇贝经不同处理得到的扇贝肉原料的细菌及相关理化特性.方法 对样品的水分含量、pH值、菌落总数、耐热菌数、大肠菌群进行测定,并对细菌类别和菌群进行了定性和定量分析.结果 鲜活扇贝经过汽蒸热处理4、6和8 min后,pH值均为6.58左右,水分含量降低较明显,并依次降低1%;菌落总数均为103CFU/g,耐热菌数均为102CFU/g,并呈依次降低趋势;大肠菌群均未检出;细菌类别均为耐热的G+杆菌和球菌;菌相比较单一,芽孢杆菌占60%左右,并呈依次增加趋势,葡萄球菌和微球菌约占1/3,并呈依次降低趋势,还残存少量棒状杆菌.综合数据表明,汽蒸处理6 min为最好的原料处理方法.企业处理原料水分含量76.80%,pH值接近中性,均偏高;菌落总数接近104 CFU/g,耐热菌数为102 CFU/g,检出大肠菌群.结论 结合生产现场考察表明,企业鲜活扇贝清洗和加热方法有待改进,加热强度应该增加.市售原料样品数据表明其处理过程不良,不宜用于软烤扇贝加工.另外分析了企业使用的调味配料、加工用水、包装材料的有关性状,发现调味配料细菌较多,可能增加控制困难.

    Abstract:

    Objective To observe the bacterial and chemical characteristics of scallop raw materials which were treated by differently ways from fresh scallop and p repared for lightly baked scallop. Method The water content, pH values, total viable count, heat-resistant bacterial count and coliforms in samp les were determined. Meanwhile, bacterial species and flora were also qualitatively and quantitatively determined. Results The pH values of scallop s steamed for 4 min, 6 min or 8 min was all about 6158. The water content of samp les decreased for 1% in turn. Total viable count ( TVC) of bacteria in samp les was 103 CFU /g. The heat2resistant bacteria count was 102 CFU /g and p resented in a reduction tendency in turn. Coliforms were not detected. The bacteria species in samp les were Gram-positive bacillus and coccus. The bacterial phaseswere much simp lex. B acillus spp. accounted for 60% and ascended in turn; S taphy lococcus spp. and M icrococcus spp accounted for one third of the total bacteria, and p resented in a reduction tendency; and Corynebacterium spp. accounted for a small percentage. Itwas indicated from the integrated data that the scallop steamed for 6 min was the bestway of p rocessing. The water content of samp les treated by enterp riseswas 76180%; pH valueswere in neutral; TVC and heat-resistant bacteria counts were 104 CFU /g and 102 CFU /g respectively; and coliformswere detected. Conclusion Based on the visits to p rocessing sites, the methods for cleaning and heat treatment of fresh scallop s needed to be imp roved. The results indicated that the p rocessing p rocedures have not been under good control and the raw materialswere not suitable for p rocessing lightly baked scallop. On the other hand, the bacteria counts in flavoring ingredients, the water for p rocessing and packing materials were many, which might increase difficulties for the quality control of lightly baked scallop.

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许钟,杨宪时,郭全友.软烤扇贝加工源头的细菌控制研究[J].中国食品卫生杂志,2010,22(1):60-64.

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