北京口岸进口鲜活海产品中副溶血性弧菌致病性分析
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R155.55 R378.3

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Detection of Pathogenic Vibrio parahaemolyticus in Imported Seafoods in Beijing
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    摘要:

    目的调查从北京口岸进口的鲜活海产品中是否存在致病性副溶血性弧菌。方法对2005年从北京口岸进口的鲜活海产品中分离的267株副溶血性弧菌(Vibrio parahaemolyticus)的尿素酶活性、神奈川现象和毒性基因(tdh和trh)进行了检测。结果267株副溶血性弧菌中27株尿素酶呈阳性,其中14株菌tdh基因和砌基因呈阳性。tdh基因和trh基因呈阳性的14株菌中有10株菌神奈川现象为阳性,并且全部分离自从加拿大进口的象拔蚌。结论北京口岸进口的鲜活海产品中存在致病性副溶血性弧菌,主要集中在象拔蚌中。应对从加拿大进口的象拔蚌加强监管,防止致病性副溶血性弧菌引起食物中毒发生。

    Abstract:

    Objective To understand the situation of contamination of seafoods by Vibrio parahaemolyticus.Methods 2?349 batches of seafood imported to Beijing during March-October 2005 were examined for V.parahaemolyticus. V.parahaemolyticus was discovered from 238(10.13%) batches. These 238 strains and the other 29 strains isolated before were further examined for the ability to hydrolyze urea, kanagawa phenomenon (KP~+) and the presence of tdh and trh genes.Results 27 strains were positive for urea hydrolysis, of which 14 strains were positive for tdh and trh genes. Among these 14 strains, 10 strains were positive for kanagawa phenomenon. All the KP~+ strains were isolated from samples of geoduck imported from Canada. Conclusion The results indicated that imported seafoods are contaminated by pathogenic V. parahaemolyticus, especially in geoduck from Canada.

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陈广全,汪琦,曾静,张惠媛,张昕,张西萌.北京口岸进口鲜活海产品中副溶血性弧菌致病性分析[J].中国食品卫生杂志,2007,(3).

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