Objective To investigate the antimicrobial resistance of S.typhimurium isolates from food in China, the twenty-three strains of S.typhimurium isolated from 2002 to 2005 within the areas of Chinese National Foodborne Dieases Surveillance Network were tested for the antimicrobial resistance and molecular types. Methods All the strains were detected the antimicrobial resistance by the disk diffusion method and molecular typed by PFGE.Results Fifteen out of 23 strains were multiple antimicrobiol resistant. Six strains (40%) were resisted to four to five kinds of antibiotics, five strains (33.3%) to six to nine kinds, four strains (26.7%) to ten. All the tested strains were typed into sixteen genotype, with the five genotypes have more than one strains.Conclusion The multiple microbial resistance of foodborne S.typhimurium strains has showing the prevalent problem. PFGE is one of the good typing methods for S.typhimurium isolates. It is also indicated that the results of the antimicrobial resistance patterns are quite closed to the PFGE typing in this study.