Objective To develop a PCR-immunochromatographic test (PCR-ICT) for screening genetically modified (GM) soybean.Method The promoter CaMV35S was used as a marker for screening genetically modified organisms (GMOs). According to the gene sequences of promoter CaMV35S introduced into GMOs, PCR primers were quoted from previous reports and the former primer was labeled with biotin, and a specific probe was designed and labeled with digoxigenin. The PCR amplification product was detected and identified by colloid gold immunochromatographic test.Result 0.5% of standard GM soybean powder could be detected by the newly developed PCR-ICT. The results of detecting soybean samples using this test consisted with those of using agar gel electrophoresis.Conclusion The newly developed PCR-ICT appears to be a rapid and convenient technique for screening GMOs. It could simultaneously detect and identify specific PCR product by DNA hybridization and colloid gold immunochromatographic test.