The compositions and content of bioactive substances in Xinjiang grapes were analyzed. Oleanolic acid, resveratol and procyanidins were extracted from grape skins, grape seeds and waste residue of grape wineries by ultrasonic wave or reflux method, and then analyzed by high-performance liquid chromatographic method or colorimetric method. The content of oleanolic acid in grape skin was about 1% and satisfied the demands of industrial production. The content of oleanolic acid in the breeds of Xinjiang local grapes was higher than that in grapes introduced from Europe. When arranged in order of oleanolic acid content: the artificial fermented skin in waste residue from wineries>the natural fermented skin in waste residue from wineries>the fresh grape skin. The content of resveratol was higher in grape skins than in grape seeds and abundant in introduced Europe grapes than in Xinjiang local grapes. When arranged in order of resveratol content: the artificial fermented skin in waste residue from wineries>the natural fermented skin in waste residue from wineries>the fresh grape skin>the stale grape skin. The content of procyanidins was higher in grape seed than in grape skin and similar between introduced Europe grapes and Xinjiang local grapes. When arranged in order of procyanidins content: the fresh grape seeds>the natural fermented seeds in waste residue from wineries>the artificial fermented seeds in waste residue from wineries. Xinjiang grapes were rich in bioactive substances such as oleanolic acid, resveratol and procyanidins, which were beneficial to human health.