The clenbuterol quick measure card, HPLC and GC MS method were used respectively to determine clenbuterol in samples of pork lung and pork lung soup from a case of food poisoning. The results of determination were positive on the quick measure card. Confirmed by spectrometry, the results by HPLC were also positive in pork lung soup and pork lung with contents 0 65?mg/kg and 0 59?mg/kg respectively, and GC MS method proved positive results as well. Each of the three methods has its specificity, and should be selected for different demands of testing clenbuterol in food poisoning samples. It also shows that we should strengthen the research on the methods of analytical examination in public health emergencies.