A high\|performance liquid chromatography method for the quantification of major phospholipid classes\|phosphatidylcholine (PC),phosphatidylethanolamine (PE) in lecithin dietary supplements was described in order to determine their effective constituent accurately and rapidly.The method precision of PC and PE ranged from 5 4% to 9 0%.The recoveries for PC and PE ranged from 81 3% to 86 0% and from 83 0% to 105 6% respectively.5 kind of lecithin dietary supplements samples were measured under the optimized liquid chromatographic condition.The content of PC and PE ranged from 15 2% to 24 2% and from 3 1% to 6 1% respectively.The method described in the present study is simple,rapid and accurate,it could be recommended as a routine analytical method.