For established a method for evaluating anti\|oxidative health food by using quantitative detection of carbonyl content in proteins.2,4\|Dinitrophenyl hydrazine colorimetry was used for detection of the protein carbonyl content in different tissues of both infant and old mice.Three kinds of anti\|oxidative health food were used for feeding different groups of mice.The experiments showed,according to the increase of age the protein carbonyl content increased significantly in the order of brain>liver>serum.So,the brain tissue was used as a sensitive material for the experiments.Two groups of mice were fed with two different kinds of foods with anti\|oxidative function for 50 days,then the brain protein carbonyl content and other anti\|oxidative index set by our country were detected.The results revealed,this experiment method can be used to reflect the protective function of anti\|oxidative health foods to proteins.The results of inspection by our method and the results by the method issued by the ministry of health were in good coordination.Using this method introduced by us may provide one more evidence for evaluating anti\|oxidative health food.