For study of the anti-fatigue effect of Ciwujia preparation, sixteen male adults' exercise tolerance were tested under increasing progressively sports, and the swimming experiment of mice had been done before. The results indicated intaking Ciwujia preparation could enhance work capacity of under-anaerobic threshold, the respiratory quotient decrease to 0.86 from 0.99 and the energy supplying from fat increase 43 percent. The detecting of continued constant carrying exercises under 450 kg.m/min of twenty university students showed that the energy supplying from fat during exercises increased 10.2 percent, the heart rate decreased 10.3 percent and the shock input of oxygen increased 9.8 percent after intaking Ciwujia preparation for a week while the energy supplying from fat increased 6.8 percent intaking the preparation only once. It proved the continous intaking better effect. The results of lactic acid, heart rate, anaerobic threshold pointed out that the preparation have anti-fatigue effect on human through increasing the capacity of oxygen input, saving muscle glycogen under either increasing progressively exercises or continuous constant carrying ones. Author's address Wu Yongning, Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medcine, Beijing 100050, PRC.