Abstract:Objective To analyze the influencing factors of Salmonella contamination， the serotype distribution and molecular typing of Salmonella isolates in meat products in Yibin city were studied.Methods A total of 29 Salmonella strains isolated from commercial meat products in Yibin city of from 2015 to 2020 were analyzed by slide agglutination test for serotype and pulsed field gel electrophoresis（PFGE） for molecular subtyping. Multivariate Logistic regression analysis was carried out on the effects of geographical distribution， sample source and sales mode on the detection composition of Salmonella serogroup.Results Fourteen serotypes and 4 serogroups were detected in the Salmonella isolates. The dominant bacteria were Salmonella typhimurium， Salmonella give and Salmonella enteritidis. The PFGE cluster analysis showed 18 genotype. The genotypes of Salmonella enteritidis were highly homologous with similarity index of 100%， indicating the same source. Salmonella typhimurium with 4 genotypes and Salmonella give with 2 genotypes both had lower similarity. Multivariate Logistic analysis showed that the risk factor on serogroup D of Salmonella was geographical distribution （OR=1.053， P<0.05）. The risk factors on serogroup E of Salmonella were sample source （OR=3.887， P<0.05） and geographical distribution （OR=1.227， P<0.05）.Conclusion The poultry breeding sites and sample source are risk factors for Salmonella infection， and the hygienic inspection should be reinforced accordingly.

Abstract:Objective To establish a rapid detection method for enrofloxacin and ciprofloxacin in milk products by surface enhanced Raman spectroscopy.Methods Five percent acetic acid was used for extraction and dichloromthane was used for liquid-liquid distribution and purification. The parameters including addition amount of colloidal silver， extraction solution and coagulant were determined by single factor experiment.Results Raman characteristic shifts of enrofloxacin and ciprofloxacin were （736±5）cm^-1， （785±5）cm^-1， （1 345±5）cm^-1 and （1 395±5）cm^-1. According to the position and intensity of probability of detection model， the detection limit of enrofloxacin and ciprofloxacin in milk was 50 μg/kg.Conclusion The method is simple in pretreatment， the detection is fast and the result is accurate. It only takes 25 min from sample pretreatment to the result issuing. It can be used for the rapid on-site qualitative determination of enrofloxacin and ciprofloxacin in milk.

Abstract:Objective Gas chromatography-mass spectrometric （GC-MS） with positive chemical ionization technique （PCI） was developed to detect nine haloacetic acids in drinking water.Methods The samples were extracted by methyl tert-butyl ether， derived by acidified methanol， and analyzed by GC-MS with PCI source under selected ion monitor mode. The matrix-matched calibration curve internal standard method was used for quantification.Results The nine haloacetic acid compounds showed good linear relationship in the range of 0.2-100 μg/L， with the correlation coefficients over 0.99. And the limits of quantification （S/N=10） ranged from 0.107 to 1.640 μg/L. The average recoveries of nine haloacetic acid compounds were 81.8%-110.2%， and the relative standard deviations were 3.9%-8.8%.Conclusion The method has good precision and sensitivity， and can be used for the determination of haloacetic acid compounds in drinking water.

Abstract:Objective To establish a high-performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） method for the detection of 6 quinolones residues in milk tofu.Methods The milk tofu sample was extracted with 0.1 mmol/L EDTA-Mcllvaine buffer with 0.1 mL each of ferrocyanide and zinc acetate， purified by HLB Pro solid phase extraction column， and separated by Shim-pack FC-ODS （150 mm×20 mm， 3 μm）， gradient eluted by 10 mmol/L ammonium acetate （containing 0.1% formic acid）-methanol acetonitrile （40：60， V/V）， and detected in full scan （full MS） mode.Results The results showed that the 6 quinolones had good linearity in the concentration range of 10-100 ng/mL， the correlation coefficient （R²） was all above 0.995， the limit of detection （LOD） was 0.5-1.5 μg/kg， the limit of quantification （LOQ） was 2-4 μg/kg， the recovery rate of spiked sample was 82.3%-101.6%， and the relative standard deviation was less than 10%.Conclusion This method is highly sensitive， rapid and accurate， and suitable for rapid screening， quantitative and qualitative analysis of 6 quinolones in milk tofu.

Abstract:Objective To develop a method for determination of chloramphenicol residue in poultry eggs （hen egg， duck egg， quail egg， and goose egg） using QuEChERS （Quick， Easy， Cheap， Effective， Rugged， Safe） protocols combined with high performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS）.Methods The chloramphenicol residue in egg samples was extracted with acetonitrile， followed by QuEChERS protocol with cleaning-up mixture （PSA∶C18=1∶1）， and then determined by liquid chromatography-tandem mass spectrometry （HPLC-MS/MS） with internal standard quantification.Results The linearity of chloramphenicol in poultry eggs was 0.020-2.0 μg/kg， with a correlation coefficient r>0.999. The limit of detection （LOD） of the method was 0.020 μg/kg， and the limit of quantitation （LOQ） was 0.050 μg/kg. Recoveries ranged from 80.8% to 119.0% at spiked level of 0.020， 0.10 and 0.50 μg/kg with the relative standard deviations （RSDs） of 4.09%-15.21% （n=6）. The developed method was validated using real-life samples as well as naturally incurred egg samples and showed good applicability in chloramphenicol residue analysis in poultry eggs.Conclusion This proposed method is rapid， simple and sensitive， which could be effectively used for monitoring and determination of chloramphenicol residue in poultry eggs.

Abstract:Objective To compare the effects and experimental conditions of membrane adsorption method， membrane adsorption-PEG method and membrane adsorption-ultrafiltration method on virus enrichment in purified water.Methods MS2 bacteriophage was used as target virus to prepare water samples with high and low concentrations， and the standard curve of reverse transcription-fluorescence quantitative polymerase chain reaction （RT-qPCR） was established for quantitative detection. Water samples were enriched by membrane adsorption method， membrane adsorption-PEG method and membrane adsorption-ultrafiltration method. The recoveries of different filtration membranes， different ultrafiltration tubes and two nucleic acid extraction methods were compared.Results The average recoveries of membrane adsorption-ultrafiltration method， membrane adsorption-PEG method and membrane adsorption method were 19.80% ± 12.19%， 12.71% ± 9.09% and 9.05% ± 4.89%. The order of influencing factors on the recoveries was： membrane adsorption-ultrafiltration method > membrane adsorption-PEG method > membrane adsorption method. High concentration > low concentration. The effect of virus enrichment of mixed cellulose ester membrane was better than nylon membrane. There was no difference in the enrichment effect of ultrafiltration tube at 100 and 50 K， and the nucleic acid extraction effect of silica gel centrifugation column method and magnetic bead method.Conclusion The enrichment efficiency can be improved by secondary enrichment. Membrane adsorption-ultrafiltration method is simple to operate， short time consuming， low dependence on equipment， and easy to be popularized. The experimental conditions of membrane adsorption-ultrafiltration can be optimized and improved to expand the application of different water bodies and different virus enrichment.

Abstract:Objective To establish a rapid detection method for adulteration of fur animal-derived components in meat products.Methods Three positive plasmids （fox， raccon dog and mink） for detection were constructed， and their specificity， sensitivity and applicability were analyzed by real-time quantitative PCR.Results The constructed positive plasmid molecules of fox， raccoon dog and mink had high specificity and sensitivity， and the sensitivity could reach 10^-4 ng/μL. The amplification efficiency of standard curves were 94.451%， 117.461% and 114.709%， respectively. And the correlation coefficient R² was over 0.995. Three components could be detected in mixed meat samples as well as common meat products， the limits of detection were less than 1%， which had good feasibility and applicability.Conclusion The three positive plasmids constructed by this method could meet the needs of meat adulteration detection in practical work.

Abstract:Objective To establish a method based on dispersive liquid-liquid microextraction coupled with gas chromatography for the simultaneous determination of seven common preservatives and three common antioxidants in vinegar.Methods Chloroform was used as the extractant and ethyl acetate was used as the dispersant. The variables affecting the extraction efficiency were investigated， such as the extractant， dispersant， salt addition and pH.Results All analytes were linear in the range of 0.004-1.00 mg/mL， and the correlation coefficients （r） were 0.998 98-0.999 98. The limits of detection were 0.03-0.10 mg/kg and the limits of quantification were 0.10-0.34 mg/kg. The recovery rates were 71.6%-93.5% and the relative standard deviations were 2.3%-8.7%.Conclusion The method is applicable for the simultaneous determination of preservatives and antioxidants in vinegar.

Abstract:Objective To establish a method for the determination of isoflavone in soybean by ultra performance liquid chromatography tandem mass spectrometry with the contents of genistein， daidzein and glycitein as the indexes.Methods The samples were extracted by ethanol-water （3+1， V/V）， separated by Acquity UPLC^? BEH C18 column（3.0 mm ×150 mm， 1.7 μm） and then eluted by methanol and 0.1% formic acid solution （containing 5 mmol/L ammonium acetate） as mobile phase， detected by UPLC-MS/MS under the electrospray ionization （ESI^-） anion ion mode with multiple reaction monitoring mode. Results The linear relationship between genistein and daidzein was good in the range of 5.0-500 μg/L （r>0.995）， the detection limits were 2.7 and 4.0 mg/kg， respectively. The average recoveries at the addition levels of 300.0， 600.0， and 1 200 mg/kg were 89.4% to 102.2% and the relative standard deviations （n=6） were 1.5% to 4.6%. The linear relationship of glycitein was good in the range of 0.5-50.0 μg/L （r>0.995）， the detection limit was 0.6 mg/kg. The average recoveries at the addition levels of 30.0， 60.0， and 120.0 mg/kg were 85.7% to 104.0% and the relative standard deviations （n=6） were 1.3% to 2.8%.Conclusion The method is simple， sensitive and accurate， which is suitable for the determination of isoflavone in soybean.

Abstract:Objective To establish a rapid， sensitive and specific detection method for 4 diarrheal bacteria （Salmonella， Shigella， Vibrio cholera and Escherichia coli O157：H7） by the Clustered regularly interspaced short palindromic repeats associated protein 13a （CRISPR-Cas13a） combined with recombinant enzyme-mediated isothermal amplification （RAA）， called RAA-Cas13a.Methods In this study， the specific primer for RAA and CRISPR RNA （crRNA） of 4 different diarrheal bacteria were designed. The sample nucleic acids were amplified by RAA， and the amplification products were then detected with CRISPR-Cas13a. Compared with real-time quantitative polymerase chain reaction（RT-qPCR）， the sensitivity and specificity of the RAA-Cas13a method were evaluated.Results The established RAA-Cas13a detection method for Shigella， Vibrio cholera and Escherichia coli O157：H7 had the detection limit of 10 copies/μL， the detection limit for Salmonella was 1 copy/μL， and each bacteria did not have cross-reaction with the other ten bacteria. Meantime， the detection of the RT-qPCR and RAA-Cas13a were highly consistent in 200 suspected samples and 40 artificial simulation samples （Kappa=0.927 and 1.000， respectively）.Conclusion The established RAA-Cas13a detection method has the advantages of high sensitivity and strong specificity. It can quickly detect and screen diarrheal diseases caused by 4 pathogenic bacteria.

Abstract:Objective To develop a method for the determination of pyroxasulfone residue in plant derived foods by QuEChERS combined with gas chromatography-triple quadrupole mass spectrometry.Methods Pyroxasulfone in rice， wheat， sorghum， corn， soybean， peanut， sweet potato， potato and sunflower seeds were extracted with ethyl acetate， and cleaned up with primary-secondary amine， stearyl bonded silica gel and anhydrous magnesium sulfate as dispersive solid-phase extraction sorbent. After centrifugation and filtration， the target compound was analyzed by gas chromatography-triple quadrupole mass spectrometry， and quantified by matrix matching external standard method. The extraction solvents， extraction method， purification method and instrument conditions were optimized. The matrix effect was investigated and the optimal pretreatment method and instrument conditions were determined.Results Under the optimal conditions， the calibration curves were linear in the range of 0.000 5-0.05 mg/L with correlation coefficients above 0.999. The recoveries were 83.8%-104.3% at four concentration levels with relative standard deviations （RSDs， n=6） of 2.6%-6.7%. The quantitation limit of pyroxasulfone was 0.001 mg/kg.Conclusion The method is simple， rapid and sensitive， and could meet the requirements for the detection of pyroxasulfone residue in plant derived foods.

Abstract:Objective An ultra-high performance liquid chromatography-tandem mass spectrometric （UPLC-MS/MS） method was developed to quantitatively determine the six kinds of aflatoxins in dairy products by employing a novel graphene based adsorbent.Methods The samples were dissolved in water and extracted by acetonitrile. After concentrated with nitrogen， the extracts were re-dissolved in the mixture of acetonitrile and 0.1% formic acid （1∶1）， and purified by graphene adsorbent. The analytes were separated by UPLC， and detected with triple quadrupole mass spectrometer.Results The linear range was 0.05-100 ng/mL with correlation coefficients （r） above 0.998. The recoveries of aflatoxins in spiked dairy product ranged from 91.0% to 118.5% with relative standard deviations of 2.39%-6.00%. The limits of quantitation were in the range of 0.01-0.05 μg/kg.Conclusion This simple and sensitive method can be used for rapid determination of six kinds of aflatoxins in dairy products.

Abstract:Objective To provide data basis for nutrition evaluation and improving measures for preschool children with food allergy， the current situation of food allergy， the eating habits and dietary characteristics of preschool children in Shanghai were analyzed.Methods A total of 5 215 preschool children aged 3-6 years old were selected by stratified cluster sampling， covering 15 kindergartens in 6 districts of Shanghai （Hongkou， Jing’an， Xuhui， Minhang， Jiading and Chongming District）. The demographic characteristics， feeding environment， food allergy and food habits was collected from children’s parents by electronic questionnaires.Results The self-reported food allergy in preschool children accounted for 16.8%. The highest proportion of allergenic food was aquatic products （including fish， shrimp and seafood etc.） （7.2%）， followed by eggs （5.3%）， milk or dairy products （4.2%）， fruits （2.4%）， nuts （2.1%）， beans （0.5%）， meat （0.5%）， flour （0.4%） and vegetables （0.4%）. Among the children with food allergy， 61.8% were allergic to one type of food only， and 24.9% were allergic to at least two types of food. Children with allergies to aquatic products and fruits tended to have a single food allergy， while those with allergies to eggs， milk and nuts tended to have a combination of two or more food allergies. Compared with children without food allergy， food allergic children had reduced consuming frequency of sensitized food but increased frequency of alternative food.Conclusion There was a high proportion of parental reports on food allergy in preschool children in Shanghai. The main allergenic food was aquatic products， eggs， milk or dairy products and fruits. Children allergic to aquatic products tended to be single-type food allergy， while those allergic to egg， milk and nut tended to be allergic to multiple foods. Children with food allergy had obvious food avoidance behavior but potentially increased the intake of alternative food.

Abstract:Objective To identify and detect the diarrheagenic virulence genes of Aeromonas hydrophila in ready-to-eat seafood sold in Macao.Methods Aeromonas hydrophila were isolated from 80 ready-to-eat seafood samples of crustaceans， fish， shellfish and cephalopods. And they were identified by real-time polymerase chain reaction based on 16S rRNA gene. Multiplex polymerase chain reaction was used to determine the diarrheagenic virulence genes in the isolated strains.Results Fifty seven of the 80 samples （71.25%） were positive with Aeromonas hydrophila. Among the positive samples， 6 of the samples （10.53%） were detected with diarrheagenic toxic genes. Among 6 strains which carried toxic genes， 4 strains carried 2 toxic genes and 2 strains carried only 1 toxic gene. Four strains carried alt （7.02%）， followed by 3 strains with aerA （5.36%）， 2 strains with ast （3.51%）， and only 1 strain with act （1.75%）. After Chi-square test and analysis， there was no direct relationship between the detection rate and 4 types of ready-to-eat seafood（P>0.05）. In addition， 13 strains of Aeromonas hydrophila were isolated from 15 cooked samples.Conclusion The high isolation rate of Aeromonas hydrophila may be due to contamination caused by improper packaging， transport and processing. The detection of toxic genes was low， but the risk cannot be ignored and integrated monitoring based on food chain is important.

Abstract:Objective The SWOT-AHP analysis model was applied to make the best strategic choice for grain inspection institutions.Methods The strengths （S）， weaknesses （W）， opportunities （O） and threats （T） faced by grain inspection institutions on the basis of SWOT framework was analyzed. The strategic quadrangle was constructed with analytic hierarchy process （AHP）.Results Based on the analysis of current situations and problems， this paper points out that the WO strategy may be the best choice， which was to overcome internal weaknesses by taking advantage of opportunities in the external environment.Conclusion The study provides a new perspective and reference to build a better grain inspection systemsin China.

Abstract:Objective To investigate and analyze the standardized management and clinical application of food for special medical purposes （FSMP） in secondary and above medical institutions of China.Methods In the form of online questionnaire， the medical institutions in 34 provinces / municipalities were sampled through the platforms of Sichuan Provincial Clinical Nutrition Quality Control Center and the Clinical Nutrition Discipline Alliance of West China Hospital.Results A total of 300 valid questionnaires were collected. In terms of FSMP management： 24.3% of medical institutions set up FSMP management committee； 64.3% of medical institutions formulated the catalogue of FSMP. Medical institutions with FSMP warehouses and FSMP preparation rooms accounted for 47.3% and 50.7%， respectively. 40.7% of medical institutions incorporated FSMP into information management； 43.3% of medical institutions charged unified coding fees. For clinical application of FSMP： 94% of medical institutions required nutrition risk screening before FSMP prescription； 84.7% of medical institutions required qualified prescribers； nearly 90% of medical institutions prescribed FSMP by nutritionists； 67.3% of medical institutions required the superior clinicians/ nutritionists to review the FSMP prescription before implementation. The adverse reaction of FSMP use was monitored in all 300 medical institutions， and only 30.7% reported the monitoring data of the adverse reactions to the provincial / municipal clinical nutrition quality control center.Conclusion The government should strengthen the construction of specialized fee catalogue and the unification of prescription qualification training； medical institutions at all levels， especially hospitals in the western region and secondary hospitals nationwide， should set up FSMP management committee as soon as possible， and integrate FSMP into the hospital information system. The clinical nutrition discipline should strengthen the formulation of clinical norms， detailed rules for the application of FSMP and supervision system. Safety monitoring and effectiveness evaluation should be strengthened so as to maximize the value of nutritional treatment of FSMP.

Abstract:Food standards are the important basis and criterion to guide the scientific， standardized and orderly implementation of food safety supervision.But there are problems such as poor compliance with regulatory needs. At present， China’s food standard system is still in the stage of gradual construction stage. According to the role and application of food standards in the food safety supervision， the compliance and matching are analyzed and studied between food standard system and actual supervision. The paper mainly analyzes China’s food standard system according with the application during food safety supervision. It is suggested to improve the follow-up evaluation of the standards tracking and evaluation gradually， simplifying and optimizing the revision process of the standard system， and integrating food classification standards to provide strong support and basis for food safety supervision.

Abstract:Objective To analyze the spatial and temporal characteristics and clustering distribution of foodborne diseases in Guangdong Province from 2016 to 2018， and provide evidence for the development of prevention and control strategies.Methods The cases of foodborne diseases in Guangdong Province from 2016 to 2018 were extracted from the National Foodborne Surveillance and Reporting System. The epidemiological characteristics of the cases were analyzed， and ArcGIS 10.8 software was applied to display the spatial autocorrelation analysis of cases.Results From 2016 to 2018， a total of 76 943 cases of foodborne diseases were reported in Guangdong Province， with a male to female ratio of 1.16∶1. The most common occupational population was scattered children （26.58%， 20 452/76 943）， and the number of cases in 0-5 years old group was the largest （23 584 cases， 30.65%）， 29 072 cases were sampled and tested， and the overall positive detection rate of pathogenic bacteria was 17.03% （4 950/29 072）. The positive rate of Salmonella was 14.84% （4 314/29 072）. The positive rate of Vibrio parahaemolyticus was 1.29% （374/29 072）. The positive rate of diarrheagenic Escherichia coli was 0.83% （241/29 072）. The positive rate of Shigella was 0.07% （21/29 072）. The overall positive rate of norovirus was 0.83% （241/29 072）. Mixed food accounted for the largest proportion of suspected source disease exposure（21.69%）， and the main place of eating was at home （65.53%）. The annual incidence of foodborne diseases in 2016—2018 generally showed two peaks： one peak was in August and the other one was from October to December. Spatial analysis showed that the incidence of foodborne diseases had a certain spatial clustering from 2016 to 2018.Conclusion The incidence of foodborne diseases was low in Guangdong Province from 2016 to 2018. Salmonella was the main pathogen detected， mainly concentrated in the 0-5 years old age group， and the peak of incidence was concentrated in August， October to December. The occupation distribution was mainly scattered children， and diarrhea was the main symptom. The suspected food was mainly mixed food， and the eating place was mainly family. The incidence of foodborne diseases in Guangdong Province has obvious spatial and temporal clustering， and the prevention and control of foodborne diseases should be strengthened.

Abstract:Objective To provide reference for the prevention， control and investigation of similar incident in the future， a poisoning incident caused by datura stramonium was investigated.Methods According to “Technical guideline for epidemiological investigation of food safety incidents” and “Diagnostic criteria and principles of management for food poisoning of datura stramonium （WS/T 3—1996）”， on-site epidemiological investigation， food hygiene investigation， laboratory testing and results determination were carried out.Results Sixty four cases were poisoned in the incident with attack rate of 60.95% （64/105）. The main symptoms were dry mouth， fatigue， headache， nausea， vertigo， dilated pupils， blurred vision and difficulty to speak. The mean incubation period was 2 h （0.5-5.5 h）. Atropine and scopolamine were detected in the suspicious food remains （yellow cakes） and the raw glutinous broom corn flour， the blood and vomit of cases. The stalks， fruits and seeds of datura stramonium were found in the glutinous broom corn planting land.Conclusion The incident was a food poisoning incident caused by the glutinous broom corn being harvested with datura stramonium. It is suggested that publicity and education should be carried out to improve farmers’ ability to identify wild toxic plants. Strengthening field management and clean up toxic plants before mechanical harvesting is critical to avoid the recurrence of such incident.

Abstract:Objective To provide scientific basis for formulating prevention and control measures， the epidemiological factors of an foodborne gastroenteritis outbreak caused by Norovirus in a university of Wuhan in December 2021 were analyzed.Methods The field epidemiological method was used to investigate. The risk factors leading to the event were studied by descriptive epidemiological method and case-control method. Anal swabs and environmental samples were collected for pathogenic detection.Results One hundred and thirty nine cases were identified， with an attack rate of 0.43%. The main clinical symptoms were nausea， vomiting and diarrhea. Eating H Street pickles was a risk exposure factor （OR=15.96， 95%CI： 2.32-383.9）. Twenty nine cases and 5 food practitioners were positive for Norovirus GⅡ.Conclusion The foodborne gastroenteritis outbreak was caused by Norovirus GⅡ. In the season of high incidence of Norovirus infection， the monitoring of norovirus infection should be strengthened in schools and food practitioners， and health education of food hygiene should be carried out actively to prevent the outbreak of intestinal pathogen infection.

Abstract:Objective To investigate the etiological features and traceability of a food poisoning incident in Pudong District， Shanghai， and to provide a basis for the future investigation and disposal of similar incidents.Methods Anal swabs of the patients and a chef and food residues were collected for pathogens isolation and identification. Enterotoxin detection immunosorbent assay （ELISA）， drug sensitivity test， pulsed field gel electrophoresis （PFGE） and multilocus sequence typing （MLST） were used for genotyping and tracing.Results Nine anal swabs from patients， one anal swab from the chef， and 3 food residues were positive for Staphylococcus aureus （S. aureus）. Ten isolates from patients and the chef carried Staphylococcus enterotoxin （SE） A and SEE， while 3 isolates from food carried SEA， SED and SEE. All the isolates were resistant to penicillin. The S. aureus strains from the case shared the same PFGE pattern and the MLST type of them belonged to ST6.Conclusion The cause of this food poisoning outbreak was S. aureus contamination. It is necessary to further strengthen the education and management of food processing enterprises， canteens， etc. to prevent similar incidents.

Abstract:Objective To evaluate the feasibility of the autoregressive moving average model （ARIMA） for predicting the monthly incidence of foodborne diseases in Jiangxi Province.Methods The ARIMA model was constructed by Python software， and the data from January to December in 2021 was used as the validation set to evaluate the prediction performance of the ARIMA model. The short-term prediction of the monthly incidence of foodborne diseases in Jiangxi Province from January to June in 2022 was carried out.Results The incidence of foodborne diseases in Jiangxi Province from 2016 to 2021 generally showed a downward trend， with the peak incidence in August each year. The best prediction model was ARIMA（1，0，0）（1，0，2）₁₂. The Bess Information Criterion （BIC） was 96.66， and the model residual was a white noise sequence （P>0.05）. The predicted incidence rate of the model was roughly consistent with the actual incidence trend， and the overall root mean square error （RMSE） was 0.656. The efficacy of the model was verified by the data in 2021. The mean absolute percentage error （MAPE） between the predicted value and the actual value was 11.25%. It showed that the model extrapolation effect was better.Conclusion The ARIMA（1，0，0）（1，0，2）₁₂ model can be used for short-term prediction of the incidence trend of foodborne diseases in Jiangxi Province.

Abstract:Objective To provide scientific guidance for consumers to choose nutritious and cheap foods， the association between street food prices and nutritional value in Wuhan was analyzed.Methods One gourmet street was randomly selected from Jianghan， Jiang’an and Wuchang District of Wuhan City， respectively. Fifty two kinds of street foods of 6 types （barbecue， frying， steaming， baking， beverage and cold food） were selected， and the prices were recorded. After analyzing the nutrient components through standard testing in laboratory and using nutrition calculators， energy prices and the average price of nutrient-rich food （NRF_6.3） index was calculated. The correlation between unit price and NRF_6.3 index was analyzed by Spearman rank sum test.Results Baked food had the highest energy density， reaching 308.41 kcal/100 g， while beverages had the lowest energy density， reaching 79.35 kcal/100 g（P<0.001）. The energy price was the highest for barbecue （22.47 yuan/100 kcal）. And the energy prices of steaming， frying， drinks， baking and cold food were 2.15， 4.88， 3.56， 3.95 and 5.61 yuan/100 kcal. The average price of NRF_6.3 index was -2.90/yuan. The food with the highest NRF_6.3 index that one yuan could buy was cold food （2.43）， and the lowest was steaming food （-11.09）. Besides， the price of street food was significant positively correlated with protein （P<0.001）， dietary fiber （P=0.014） and fat （P=0.001）， and significant negatively correlated with added sugar （P<0.001）. There was no rank correlation between the unit price and its NRF_6.3 index among 6 types of foods （P>0.05）.Conclusion The overall price of street food in Wuhan is relatively high with high energy density， while the price of NRF_6.3 index is low. This results suggest that， for the same price， the recommended nutrient content of street food is not high， and the nutritional value is low， which does not meet the requirements of a healthy diet.

Abstract:Objective To investigate the breakfast behaviors and influential factors in primary and secondary school students in poor rural areas of Hubei Province， and provide scientific basis for carrying out nutrition improvement plan for rural students in 2019.Methods Based on stratified random cluster sampling method， 2 primary and 2 secondary schools were selected based on 3 food supply modes （school， outsource， and family） in 2 key monitoring counties of Hubei Province covered by nutrition improvement program for rural compulsory education students. One class with around 40 students were randomly extracted from each Grade from Grade 3 to Grade 9. Background information and breakfast behaviors were collected through questionnaire survey.Results The proportion of primary and secondary students having breakfast every day was 79.7% （1 273/1 598）， and it was higher in the primarystudents than in the secondary students （83.9%，841/1 002 vs. 72.5%，432/596， P<0.01）. The proportion of breakfast food reaching 3 kinds or more was only 18.8% （301/1 598）， it was higher in the primary students than in the secondary students （21.4%，214/1 002 vs. 14.6%，87/596， P<0.01）， and it was higher in the boys than in the girls （21.3%，170/800 vs. 16.4%，131/798， P<0.05）. The proportion of primary and secondary students who consumed cereals and potato， meat and eggs， milk and bean， fruit and vegetables was respectively 89.9% （1 436/1 598）， 35.4% （565/1 598）， 28.0% （448/1 598） and 29.7% （475/1 598）. The proportion of breakfast having cereals and potato， meat and eggs in primary students was less than that in secondary students （χ²=32.808 and 15.405， P<0.01）； and the proportion of breakfast having milk and bean， fruit and vegetables in primary students was higher than that in secondary students （χ²=19.241 and 40.400， P<0.01）. The results of multi-factor analysis showed that day students， girls and primary students had a higher risk of insufficient breakfast food categories than resident students， boys and primary students.Conclusion The breakfast consumption rate of primary and secondary students in poor rural areas of Hubei Province was relatively low， and the nutrition quality of breakfast was poor， especially for day students， girls and primary students. Therefore， it is necessary to strengthen nutrition education and improve the frequency and quality of breakfast for primary and secondary students in poor areas.

Abstract:Objective To evaluate the effect of nutritional health knowledge， attitude and practice theory on seafarers’ health education intervention.Methods A total of 628 seafarers who underwent health check-up in two designated hospitals in Zhoushan in 2019 were selected as subjects. They were divided into two groups by cluster random sampling method. Nutritional health intervention with different methods was conducted for one year， followed by another health check-up in 2020 and 2021. Dietary nutritional status survey was carried out in both groups during physical examination.Results After intervention， the daily intake of grain， potato， mixed beans， vegetable， fruit， soybean and nut in the intervention group was significantly higher than that in the control group， while the daily intake of edible oil in the intervention group was significantly lower than that in the control group （P<0.05）. The daily protein intake of sailors in the intervention group was significantly higher than the control group （P<0.05）. The abnormal detection rate of physical examination results after intervention was 76.0%， significantly lower than that before intervention （92.0%） （P<0.05）. Topsis comprehensive evaluation showed that the Ci value of the intervention group was significantly higher than that of the control group and closer to 1 （P<0.05）.Conclusion Dietary nutritional status and physical examination results of seafarers can be improved by nutritional KAP intervention. Nutritional KAP intervention can effectively protect seafarers’ health.

Abstract:Listeria monocytogenes is a common foodborne pathogen widely exists in the environment. Sepsis， meningitis and mononucleosis are the cardinal symptom after Listeria monocytogenes infection， also leads to abortion， fetal death in utero and neonatal death. The pathogenicity of Listeria monocytogenes is closely related to its virulence genes and virulence islands， and its pathogenicity mechanism is the result of the participation of many virulence factors and the regulation of the complex network of regulatory factors. This review aims to explain the virulence genes and pathogenesis of Listeria monocytogenes.

Abstract:In recent years， stable isotope technology has been widely used in product quality control and authenticity identification. This paper summarizes the research progress of this technology in food safety traceability and authenticity identification of various foods in recent years， looks forward to its application in China， and hope to provide reference for the administrative authoritives.