Abstract:This study aimed to describe the prevalence and the characterization of resistance mechanisms of ciprofloxacin resistant Escherichia coli isolated from food and patients in China.Methods Ciprofloxacin resistant Escherichia coli isolates were selected from 645 isolates in ciprofloxacin containing plates. Antimicrobial susceptibility testing, phylogenetic analysis, quinolone and blaCTX-M resistance mechanisms were detected by polymerase chain reaction and sequencing were carried out. Results Totally, twenty one (3.3%) ciprofloxacin resistant Escherichia coli isolates were identified. Point mutations in topoisomerase encoded genes gyrA, parC and parE were confirmed, and all isolates carried plasmid-mediated quinolone resistance genes (PMQR), including oqxA, oqxB, qnrS, aac(6′)-Ib-cr and qepA but qnrA, qnrB, qnrC and qnrD were not detected. All 21 ciprofloxacin resistant isolates also harbored blaCTX-M genes.Conclusion The isolates from food and patients showed complicated and diverse quinolone resistance mechanisms and the possible transmission of PMQR may pose potential risk to public health. In order to investigate aspects of transmission between food and community-acquired strains and to provide a scientific basis for humans, the continuous surveillance of multidrug-resistant Escherichia coli should be carried out.

Abstract:The project was carried out to study the contamination levels of Norovirus in oysters in Beijing city.Methods The Norovirus was extracted from digestive glands by phosphate buffer containing proteinase K. The RNA was extracted and purified with virus RNA extraction kit. Norovirus RNA was detected using Taqman-based one-step real time reverse transcription-polymerase chain reaction (real time RT-PCR) and quantitative analysis was performed. ResultsThree hundred and fifty-six oyster samples were detected. Fifty-seven samples were positive, including twelve GGI positive samples alone, thirty-nine GGII positive samples alone and six samples of GGI and GGII positive both. The range of the quantity of Norovirus in positive samples was 3.7×10³-2.8×10⁵ gene copies/g(digestive gland).Conclusion The oysters sold in Beijing were contaminated by Norovirus. The monitoring of Norovirus in oysters should be strengthened and risk assessment of Norovirus should be carried out to ensure safety and health of consumers and reduce the burden of disease caused by norovirus that causes diarrhea.

Abstract:To study the distribution characteristics and correlation of the pathogenicity island of the foodborne Salmonella.Methods The Salmonella isolates were collected from commercial food, commercial raw chicken, and food poisoning samples. The serotype of Salmonella was determined by serology agglutinating method. SPI gene core protein genes SPI1-SPI5 of Salmonella were detected by polymerase chain reaction. The data were statistically analyzed. Results Among the 152 isolates, Salmonella enteritidis was the most common serotype. There were different pathogenicity island type spectrums from different sources. The highest rate of SPI1 was detected in food poisoning strains. SPI1 carrying rate of raw chicken isolates was significantly higher than other commercially foods.Conclusion Salmonella pathogenicity island type spectrum distribution related to the strain of different sources, pathogenicity island gene SPI1 was positively correlated with the food poisoning caused by Salmonella. There is a potential risk of food poisoning caused by raw chicken.

Abstract:Through the correlation analysis of the main elements of paddy in China in 2015, this paper could provide reference for the data mining of food safety risk monitoring result.Methods The correlation of major elements in paddy were analyzed by Bayesian network, and the elements were grouped according to the results. Canonical correlation analysis between the grouped elements was carried out by R statistics software. Results Bayesian network found that there were direct or indirect correlations among the contents of two groups. The first typical correlation coefficient between the two groups of elements was 0.84 according to the canonical correlation analysis. The negative coefficient of V in the first typical variable was the highest, and the coefficients of Al and TI are high, and negatively correlated with the Al content, while positively correlated with the TI content.Conclusion Bayesian network and canonical correlation analysis could analyze the correlation among the major elements in paddy.

Abstract:To construct and compare the methods for correlation analysis of different elements in wheat, including barium, vanadium, cadmium, lithium, aluminum, manganese, lead, copper, selenium, chromium, and arsenic. Methods Pearson correlation coefficient and partial correlation coefficient were applied and compared to analyze the correlation among the 11 elements in wheat. Results Both of the methods could find the correlations among various contaminants from the data and had their own characteristics on computational complexity, information reflection and other aspects:Pearson correlation coefficient method need less calculation, but also reflected less information; partial correlation coefficient reflected more information but need more samples and computing resources. Under the current data and software and hardware conditions, the correlation analysis of the partial correlation coefficient was proposed.

Abstract:To research the antagonizing effect of lycopene on alcohol-induced liver injury due to continuous ethanol intake in mice.Methods A total of 100 healthy adult of SPF male Kunming mice were randomly divided into 5 groups, including the control group, the model group, and the low, middle and high dose lycopene groups with different doses (0.33,0.67,2.00 g/L) of lycopene respectively by oral gavage at 0.2 ml/10 g once a day for 30 days, and the control group and model group were given corn oil. From the 31st day, 3 hours after administration, the control group was given distilled water, while the other 4 groups were given 56 degrees liquor by oral gavage at 0.12 ml/10 g once a day for 8 days. After the last administration, 5 groups were fasted for 16 h, then triglyceride (TG), alanine aminotransferase (ALT), aspartate aminotransferase (AST), protein carbonyl content in blood serum, and malonaldehyde (MDA), superoxide dismutase (SOD), glutathione perioxidase (GSH-Px) in live were tested, and mortality and cumulative survival time were calculated. Results Compared with the control group, the TG, ALT, AST, protein carbonyl and MDA level of the model group increased significantly (P<0.01). Compared with the model group, the mortality of middle and high dose groups was significantly reduced (P<0.05), and the cumulative survival time was extend. The SOD and GSH-Px levels were significantly higher (P<0.01), while the TG, ALT, AST, MDA and protein carbonyl content were obviously reduced (P<0.05, P<0.01). With the increasing lycopene doses, the TG, ALT, AST, MDA and protein carbonyl content were dropped, while the SOD and GSH-Px levels were on the rise.Conclusion Lycopene had a certain antagonizing effect on alcohol-induced liver injury in mice.

Abstract:To explore the effects of different vegetable oils, frying time and frying temperatures on trans fatty acids content in fried flour products,with a view to determining the suitable processing conditions.Methods Palm oil, soybean oil, sunflower oil and hydrogenated palm oil were selected as four kinds of frying oil for flour products. The acid-catalyzed hydrolysis method was adopted to extract oil from flour products. Gas chromatography was used to determine the content of trans-9-octadecenoic acid, trans-11-octadecadienoic acid and trans-9,12-octadecadienoic acid at different frying times and temperatures. Results At lower temperature (160 ℃), the trans fatty acids were not detected in fried flour products with palm oil, soybean oil and sunflower oil, the oil content and trans fatty acid content of fried flour products with hydrogenated oil was significantly higher than the other. On the contrary, the index of palm oil was the best and more suitable as a frying oil. Elevated frying temperature and prolonged frying time would increase the content of trans fatty acids in flour products, and the effect of frying temperature was more obvious. Conclusion Choosing a suitable frying oil, lowering the frying temperature and time would have a positive impact on the content of trans fatty acids in flour products.

Abstract:A analytical method was developed to determine bisphenol A(BPA), bisphenol F(BPF)and their chlorinated products in drinking water using ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Methods Water samples were concentrated and purified with PLEXA solid-phase extraction catridges. The target analytes were separated on a BEH C₁₈ column(2.1 mm×100 mm,1.7 μm)with gradient elution using acetonitrile and water, and identified under negative electrospray ionization. The matrix-matched standard calibration curves were used for the quantitative analysis. Results Good linearities were achieved for all analytes with correlation coefficient (r²) above 0.99. The quantitation limits of the method (LOQ) were in the ranges of 0.01-4.00 ng/L. The recoveries of the target analytes ranged from 78.8%-99.6%, with relative standard deviations (RSD) less than 12.2%. Forty drinking water samples were analyzed by the developed method, and the detection rate of BPA, BPF and 4Cl-BPA were 45.0%(18/40),40.0%(16/40)and 32.5%(13/40), the concentrations were 0.60-9.40 ng/L, ＜LOQ-106.20 ng/L and

Abstract:An analytical method based on high performance liquid chromatography-tandem mass spectrometry was developed for the determination of perchlorate in dairy products.Methods Samples were extracted with 1% acetic acide-acetonitrile solution(1∶2,V/V), and the extract was then cleaned up on an solid-phase extraction(SPE)cartridge and separated on a IC-PakTM Anion HR column(4.6 mm×75 mm,6 μm) by using acetonitrile and ammonium acetate solution(60∶40, V/V) as mobile phase. The analytes were determined by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS) with electrospray ionization source in multiple reaction monitoring(MRM). The internal standard calibration was used for quantification. Results Good linearity was observed in the range of 0.1 to 10.0 μg/L. The limit of quantitation of the established method was 1.0 μg/kg in milk and 3.0 μg/kg in milkpowder. The recovery rate of perchlorate was 87.6%-122.0% at three spiked levels in deferent samples, with relative standard deviation of 2.0%-10.0%.Conclusion The method was sensitive, accurate, and suitable for the analysis of perchlorate in dairy products.

Abstract:To improve the high performance liquid chromatography method for quantitation of salidroside in health food.Methods The solvent and chromatography condition were optimized according to the determination of standard and extracted samples. Results Salidroside content exhibited a linear relation within 1.305-130.5 μg/ml using 50% methanol as solvent. The regression equation was y=7.377395×10-5x+0.1873961 (r=0.999 9) and the detection limit was 0.015 mg/g. Furthermore, the average recoveries were 92.1%-98.4%, and the RSD was 1.98%-3.26%. Conclusion This method was simple, accurate and reliable for determination of salidroside in health food.

Abstract:To establish a reliable pretreatment method for β-agonists determination in pork and pork liver with high performance liquid chromatography-tandem mass spectrometry (HPLC/MS-MS) assay.Methods Animal tissue was treated with β-glucuronidase/aryl sulfatase enzyme, purified by solid phase extraction (SPE), and concentrated after elution for HPLC-MS/MS analysis. Experiment conditions on solid phase extraction were optimized. Results Two β-agonists showed good linear relationship within the range of 0.5-10.0 μg/L, and the correlation coefficient was above 0.999. The samples were purified by solid phase extraction column, and the recoveries of ractopamine were 81.4%-121.0% with MCX, 97.0%-113.4% with SCX. The relative standard deviations for different matrix were 5.9%-16.2% for salbutamol and 6.4%-20.2% for ractopamine. The detection limits of salbutamol and ractopamine were 0.13 and 0.14 μg/kg respectively. Conclusion SPE methods were optimized for HPLC-MS/MS detection. The precision and recovery was suitable for the detection of animal origin β-agonist residues.

Abstract:To investigate the virulence genes, biochemical typing and multilocus sequence typing (MLST) distribution of Bacillus cereus from different sources in Wenzhou.Methods 127 strains of Bacillus cereus were identified and biochemical typed according to GB 4789.14-2014. Ten virulence genes were detected with polymerase chain reaction (PCR). Genetic typing was conducted with MLST.Results Of 127 isolates, NheA, NheB and NheC genes were accounted for 94.5% (120/127) and hblA, hblC and hblD genes were accounted for 9.4% (12/127). The emetic toxin gene was detected in 10 isolates (7.9%). Except 10 isolates which could not be typed, the rest 117 isolates were classified into type 2 (0.8%), type 5 (3.9%), type 8 (1.6%), type 9 (63.8%) and type 10 (22.0%) by biochemical typing. Seventy-two isolates could be classified into 28 sequence types (STs). ST26 (18.1%), ST144 (15.3%), ST92 (6.9%) and ST164 (6.9%) were the common STs. Cluster analysis on 28 STs generated four MLST groups and 13 singletons.Conclusion The positive rate of virulence genes was high in Bacillus cereus isolated from different sources in Wenzhou, and the genetic relationship was diverse.

Abstract:To establish a method for qualitative and quantitative determination of canthaxanthine and β-Apo-8'-carotenoic ethyl ester in eggs.Methods The analytes were extracted from the samples with acetonitrile. After cleaned up with silica SPE column, samples were analyzed by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) with an electrospray interface in positive ionisation mode. The validation of the method consisted of limit of determination (LOD), limit of quantitation (LOQ), precision and recovery. Results The LODs for canthaxanthine and β-Apo-8'-carotenoic ethyl ester were 1 and 10 μg/kg, and the average recoveries were in the range of 72.2%-91.9% with the relative standard deviations(RSD) of 4.6%-7.1% (n=5). Conclusion The results indicated that this method was simple, rapid, sensitive and suitable for the determination of canthaxanthine and β-Apo-8'-carotenoic ethyl ester in eggs.

Abstract:To establish a method for the determination of inorganic and organic selenium in food by high performance liquid chromatography-inductively coupled plasma mass spectrometry(HPLC/ICP-MS).Methods Inorganic selenium was extracted by ultrapure water. Dialysis effect was used to remove sample matrix, inorganic selenium was separated and detected by anion exchange chromatography-inductively coupled plasma mass spectrometry. Results The detection limit of Se(IV) and Se(VI) were 6.67 and 9.95 μg/kg, the recoveries were 70.2%-108.1% and 73.2%-116.9%.Conclusion This method could be used for the determination of inorganic selenium and organic selenium in selenium-enriched food.

Abstract:By analyzing a foodborne disease outbreak in a catering service, the applicability, operability and limitations of the Food Safety Law were discussed. Methods To describe, analyze and discuss the case investigation process and the court decision. Results The Food Safety Law has some limitations in the implementation, and needs to gradually improve.Conclusion The Food Safety Law needs to be improved, especially in terms of the punishment on catering service units during food borne illness outbreak, and the principles and standards of foodborne disease diagnosis needs to update.

Abstract:The purpose of this study was to learn the status of carbonated beverage consumption across age, gender, region and income categories among the population over the age of 3 in nine provinces in China.Methods Surveys were conducted among the population over the age of 3, who were selected from seven cities by stratified multistage cluster random sampling. Logistic analysis was used to analyze the influential factors. Results The consumption rate of target population was 24.2%, and the average daily consumption was 13.8 ml/d. Meanwhile, the average daily consumption was 172.9 ml/d among consumer group. The consumption of men were significantly higher than women (P＜0.01), which was 16.9 and 13.8 ml/d respectively; urban consumption was higher than rural areas (P＜0.01), which was 20.3 and 7.8 ml/d respectively; 15-17 age group had the highest consumption of 28.6 ml/d (P＜0.01), and age groups over 60 had the lowest consumption of 2.7 ml/d (P＜0.01).Conclusion The consumption of carbonated beverage manifested an increasing trend in China. Carbonated beverage consumption varied depending on age, gender, region, occupation and education level.

Abstract:To investigate the Clostridium perfringens contamination in cooked meat in Zhengzhou, and provide technical support for the safety control of Clostridium perfringens during the storage of cooked meat.Methods Total 11 kinds of samples were detected in accordance with the GB 4789.13-2012. In addition, VITEK identification method and molecular biology method (PCR molecular biology method) were used for further validation. Results In the 259 tested samples, Clostridium perfringens were detected in 38 samples. The total detection rate was 14.7%, the detection rate in different foods ranged from 0% to 33.3%, and the detection rate in salt baked chicken was the highest(33.3%,7/21). However, there was no Clostridium perfringens in stewed half duck and stewed chicken liver. The count of Clostridium perfringens in barbeque chicken, roasted chicken and stewed chicken leg have reached 5.0log₁₀ CFU/g, and the count of Clostridium perfringens in other samples were between 4log₁₀ and 5log₁₀ CFU/g.Conclusion The Clostridium perfringens contamination in cooked meat in Zhengzhou was serious. Furthermore, there was a higher possibility of food poisoning in barbeque chicken, roasted chicken and stewed chicken leg.

Abstract:Air catering means the food served to passengers and cabin crew on an airplane, which will be stored after a period of time before consumed. The microbial control and some other measures are different between air catering and ordinary food. In this paper, comparative analysis between China standard and the international standards is performed and shows that the requirements in domestic air catering standard such as temperature control, shelf-life and microbial limits are consistent with the international standards. The suggestions for implementation and revision are discussed.

Abstract:To put forward suggestions for improvement for the national food safety standard on honey through analyzing domestic and international honey standards.Methods Comparison of national and international honey standards and analysis on the sample test data were used to discuss the key indicators of current national safety standard. Results The safety standard on honey in China was in accordance with the international standards, however certain requirements need to be updated according to actual situation.Conclusion The moisture content and indicative microorganism limit were suggested to amend based on test data.

Abstract:In order to provide the basic data for further risk assessment, the levels of lead (Pb) and cadmium (Cd) in the commercial aquatic products of Guangdong were investigated.Methods Simple random sampling method was employed to determine the sample size, and 60 aquatic products were collected in each of the 21 cities and Shunde District, Guangdong Province. The concentrations of Pb and Cd were measured using GB 5009.12-2010 and GB/T 5009.15-2003, respectively. Results A total of 1 326 aquatic products were collected from 2010 to 2014. The mean level of Pb in aquatic products was 0.040 mg/kg, and the over-limit rate was 0.15% (2/1 326). The average level of Cd in aquatic products was 0.178 mg/kg, and the over-limit rate was 5.35% (71/1 326). During this five years, the small variation of the over-limit rate of Pb was found in the aquatic products, while the difference was significant for Cd (P＜0.05). For the variety of aquatic products, only freshwater fish was fully qualified, and the descending order of the over-limit rate was crab＞shellfish＞shrimp＞marine fish. The single factor pollution index (Pi) showed that the levels of Pb and Cd in the aquatic products were in the range of the background value except for the levels of Cd in shellfish and crab, while the order of heavy metal pollution index (MPI) was crab＞shellfish＞marine fish＞shrimp＞freshwater fish.Conclusion The contamination levels of Pb and Cd in aquatic products were not high, but the over-limit rate of Cd in crab could not be ignored.

Abstract:To understand the situation of formaldehyde residues in some foods from Guangzhou City, and provide reference basis for risk assessment and government supervise.Methods Food sanitation section had collected 1 416 samples for formaldehyde testing at different times in catering industries, meat markets, supermarkets and wholesale markets. Results The range of content was 2.0-4 500.0mg/kg, 13.21% (187/1 416) of the samples were above 100 mg/kg, and the proportion for poultry by-products was 32.63% (31/95), the proportion for animal by-products was 31.54% (94/298), the proportion for fish (whitebait, harpadon nehereus, fishskin) was 30.85% (29/94), and the proportion for crustaceans (shrimp meat) was 19.35% (24/124). The samples from meat markets and wholesale markets had more formaldehyde than the samples from catering industries and supermarkets. The samples collected from September to November had more formaldehyde than the other months. The samples from 2012-2013 had more formaldehyde than the other years. There was significant difference between different species, different sampling sites, different months and different years.Conclusion Some foods had a high level of formaldehyde contamination, such as animal by-products (omasum, beef heart), poultry by-products (goose intestine, duck intestine), crustaceans (shrimp meat) and fish (whitebait, harpadon nehereus, fishskin). It's relatively more serious from September to November, and the government should strengthen the supervision and prevent foodborne disease.

Abstract:To investigate the contamination of Norovirus in shellfish (oysters, mussels, scallops, clams, clam), vegetables (seedling vegetables, lettuce), berries (strawberries, blueberries), ready-to-eat seafood (salmon, shrimp ) from markets in Beijing.Methods Pretreatment methods for shellfish, vegetables and berries samples were referred to ISO/TS 15216-1; pretreatment method for shrimp and shellfish was referred to shellfish method; pretreatment method for salmon was referred to improved vegetables method. Viral RNA extraction was referred to Roche High pure viral RNA Kit K methods. Real-time reverse transcription-polymerase chain reaction testing and GI-GII typing were carried out by Roche LightMix noroviurus GI-GII in Lightcycler 480 II; Norovirus positive samples was amplified using the prime of JV12, JV13 and the amplified DNA fragments were sequenced for subtyping. Results 478 shellfish (40 positive), 62 vegetables (1 positive), 84 berries (0 positive) and 51 raw salmon (1 positive) were detected. 35 positive samples were successfully amplified by sequence analysis. 27 samples were GII.17,1 sample was GII.12, and 1 sample was Hawaii.calicivirus.Conclusion Through the norovirus monitoring in Beijing market, shellfish presented obvious seasonal distribution and higher detection rate which suggested that the shellfish was the main sources of Norovirus in food.

Abstract:To investigate the status of the bacteria contamination in student meal in Yunnan, and compare the contamination among 10 kinds of food.Methods The national standard methods about hygiene indexes and common foodborne pathogenic bacteria had been implemented on student meal from 16 representative cities in Yunnan Province in 2014 based on Food Safety Risk Monitoring Plan in Yunnan in 2014,and data were analyzed with SPSS 19.0 software. Results The unqualified hygiene indexes was 22.96% (186/810) in 810 student meal samples from unpacked food. The overall detection rate of main pathogenic bacteria was 17.53% (142/810), while it had difference between student breakfast 21.50% (63/293) and student lunch 15.28% (79/517) (P<0.05). The unqualified rates of Bacillus cereus was 15.47% (108/698) in 698 student meal samples, and 20.48% (51/249) for rice and flour foods, 13.38% (57/426) for packed lunch. The detection rate of Salmonella was 0.49% (4/810), mainly from packed lunch (0.70%, 3/430) and rice and flour foods (0.40%, 1/249). The detection rate of Staphylococcus aureus was 3.09% (25/810) with the highest rate in egg and egg products (4.55%, 1/22), followed by rice and flour foods (3.61%, 9/249), packed lunch (3.49%, 15/430). The detection rate of Listeria monocytogenes was 0.62% (5/810), mainly from packed lunch and rice and flour foods with the detection rates of 0.80% (2/249) and 0.70% (3/430). Diarrheogenic Escherichia coli were not detected. The detection rates of four kinds of foodborne pathogenic bacteria had no difference in different sampling sites (P>0.05). Except Staphylococcus aureus, the detection rates had no difference between in four quarters in 2014.Conclusion The 10 kinds of student meal with unpacked condition in Yunnan were seriously contaminated, indicating the risk of foodborne diseases. Considering the vulnerability of young students and the complexity of the food chain, food safety supervision, monitoring and management should be strengthened.

Abstract:To analyze the content of dibutyl phthalate and bis(2-ethylhexyl) phthalate in beverage and white spirits, and assess the risk of dietary exposure of residents in Yantai City.Methods A total of 385 samples were collected, the determination and quantification were performed by gas chromatography-mass spectrometer(GC-MS)in selected ion monitoring (SIM) mode. The dibutyl phthalate and bis(2-ethylhexyl) phthalate exposure was estimated by the food consumption data. The health risk was assessed by comparing the exposure with tolerable daily intake (TDI). Results The concentration of dibutyl phthalate and bis(2-ethylhexyl) phthalate was ND-4.106 and ND-3.285 mg/kg. In accordance with the mean value of food consumption estimates, the dibutyl phthalate and bis(2-ethylhexyl) phthalate exposure of local residents were 0.038 and 0.031 μg/kg BW, which were below the TDI.Conclusion The risk of dibutyl phthalate and bis(2-ethylhexyl) phthalate was low from beverage and white spirits for Yantai City residents.

Abstract:To identify the causes and suspected risk factors of the food poisoning incident, and analyze the drug resistant profile.Methods Combined with clinical characteristics and time of disease, the suspicious meals were identified, suspected food and case samples were collected for laboratory test, and pulsed-field gel electrophoresis (PFGE) and homology analysis was carried out on positive samples. Results A total of 81 cases were confirmed. The symptoms included fever ≥38 ℃ (100.00%, 81/81), abdominal pain (97.53%, 79/81), diarrhea ≥3 times (100.00%, 81/81), vomiting (69.14%, 56/81), etc. The consumption of egg fried rice contaminated by egg shell was a risk factor. The chef poured egg on the fried rice without fully heated, which leaded to the contamination. Stool samples and food solates were all positive for Salmonella enterica, and the PFGE patterns were the same. The similarity between case samples and smear samples from the egg shell was 96%. The strain was sensitive to cefotaxime, ciprofloxacin and TMP/SMZ, but was nalidixic acid-resistant. Conclusion Consumption of fried rice contaminated by Salmonella enterica was the main cause of the food poisoning, it was recommended to strengthen the supervision and management on institutional catering chef, standardize operational processes and to improve the health and safety awareness to prevent similar incidents.

Abstract:The non-intentionally added substance (NIAS) migrated from food contact material is receiving growing concern for their potential negative impact on food safety. Since the source of NIAS is complex and most of them are unknown, it poses challenges for safety assessment of food contact material, as well as market supervision. Based on the principal of threshold of toxicological concern (TTC), combined with application of Cramer decision tree, chemical analysis and bioassay screening measures, an effective and novel approach can be established to perform screening and safety assessment of NIAS from food contact material. It is, therefore, suitable for safety assessment on NIAS which lacks of toxicology data and at low exposure level. Using tier approach, it could be identified which compounds that actually pose health concern and need to be further investigated, in practical, only those compounds that TTC value exceeds 1.5 μg/kg BW have safety concern and risk assessment need to be carried out. This new strategy allows assessing high concern substances in food contact material, making transition from traditional approach that based on detection limit to a new approach base on TTC application. That will minimize unnecessary animal experiment and save huge amounts of resources on human, time and financial expending, and accelerate the process of assessment on compounds with low exposure, as well as to enhance the efficacy of market supervision.