Abstract:Objective To elucidate the natural occurrence of multi-mycotoxin in cereal and cereal-based products collected from parts of China in 2010.Methods A total of 650 corn and corn product,wheat flour,rice and peanut samples collected from Anhui,Yunnan,Fujian,Gansu,Guangxi,Hainan,Heilongjiang,Hubei,Hunan,Jiangxi,Shanxi and Shanghai provinces(cities/autonomous regions) in 2010 were analyzed for 14 kinds of mycotoxin by ultra performance liquid chromatography-tandem mass spectrometry.Results Corn produ...

Abstract:Objective To evaluate the safety of apigenin,so as to provide scientific support for the reasonable use and development of apigenin.Methods According to the procedure and methods in the Food Toxicological Safety Evaluation,a series of toxicological studies on the ingestion of apigenin were conducted,including acute toxicity,genetic toxicity as well as sub-chronic toxicity experiments.Results For the acute toxicity,the maximum tolerated dose(MTD) was higher than 8 g/kg BW.No apigenin-related gene..

Abstract:Objective To study the molecular typing of Laribacter hongkonggensis by pulsed-field gel electrophoresis(PFGE),and to find out the relationship of 7 strains of L.hongkonggensis isolated from fishery products in Guangxi on 2005.Methods Total DNA of each isolate was digested with restriction enzyme Not I,and the DNA fingerprints were obtained by running PFGE.These fingerprints of different stains were compared and their relationship was investigated by BioNumerics 5.1 clustering.Results Seven stra...

Abstract:Objective To obtain the basic data of aluminum exposure from flour products in Shanghai residents,and to estimate its health risks.Methods For the assessment of aluminum exposure from flour products in Shanghai residents,the probabilistic estimate method recommended by WHO/FAO on "Principles and methods for the risk assessment of chemicals in food" was applied.Monitoring the concentration of aluminum in flour products combined with a survey on flour products intake of Shanghai residents was cond...

Abstract:Objective To understand the dietary intake and cadmium exposure of residents in Minhang District,and to assess the safety of diet.Methods Using selective study of food categories,combining the data of food consumption survey in Shanghai residents in 2009 with the data of monitoring food pollutant in eight categories of food in Minhang District(2007-2010) to obtain the exposure of cadmium from diet in different population and to evaluate the safety of diet.Results(1) Cadmium was detected in 119 o...

Abstract:Objective To develop a method for the determination of epichlorohydrin(ECH) residues in water from teabag by liquid-phase microextraction-gas chromatography-mass spectrometry(LPME-GC-MS).Methods Epichlorohydrin in teabag water was enriched by hollow fiber liquid-phase microextraction method,separated by gas chromatography and determined qualitatively and quantitatively by mass spectrometry.Results The limit of determination was 0.18 μg/ml and the linear ranges were 0.36~7.30μg/ml(r2=0.999).Takin...

Abstract:Objective To determine the first-order structure of a recombinant beefy meaty peptide(BMP) by using electrospray ionization mass spectrometry(ESI-MS).Methods Ionization mode:electrospray ionization;detection mode:positive ion;range of ion mass scanning m/z 150~1 000;flow rate of drying gas 5 arb;nebulizer pressure 35 arb;voltages at the outlet of capillary 4.5 kV;fragmentation voltages 100 V;collision induced dissociation voltages 35%;the flow of collisions gas was controlled by electronic flow ...

Abstract:Objective To establish and evaluate the procedure for preparation of lyophilized(freeze-dried)Vibrio parahaemolyticus(VP) composed with Vibrio minicus(VM) culture materials for Proficient Test(PT) on bacteria identification.Methods Selecting a suitable proportion and concentration of VP and VM for identifying the dominant ratio of VP to VM cultured in the identification medium after lyophilization.Assessing and identifying the homogenicity of random PT specimen and evaluating the stability of sp...

Abstract:In order to understand the capability of laboratories in detecting ethyl caproate in liquor,a proficiency testing was organized in 2010;most of laboratories have got satisfactory results.It was found in this proficiency testing that the detection error would be increased if the peak area of ethyl caproate of internal standard was different from that of the tested specimen.Two laboratories have acquired desirable results by adding an internal standard according to the practical situation.Another ...

Abstract:Objective To investigate and optimize the experiment conditions of determining lead and cadmium with antimony film-plated electrode.Methods Antimony film-plated gold electrode was prepared by in-situ plating for the determination of trace lead and cadmium by differential pulse stripping voltammetry.Results Lead and cadmium displayed well defined peaks in the antimony film-plated electrode by differential pulse stripping voltammetry;and the stripping potential were-0.58 V and-0.81 V for lead and ...

Abstract:Objective To determine 15 kinds of industrial dyes simultaneously in condiments with high performance liquid chromatography(HPLC).Methods Samples were separated on a CAPCELL PAK C18 MG(250 mm×4.6 mm,5 μm)chromatographic column and gradient eluted with nitrofuran-acetonitrile-20 mmol/Lammonium acetate and detected at 450,530 and 600 nm.Results The calibration curve showed good linearity in the rang of 2~50 μg /ml,and the correlation coefficients were better than 0.99.The limits of detection were ...

Abstract:Objective To establish a method for the determination of glucosamine hydrochloride in health food by HPLC.Methods Samples were extracted ultrasonically with water and separated on a CAPCELLPAKCR column(4.6 mm×150 mm,5 μm);the mobile phase consisted of 25 mmol ammonium acetate+acetonitrile(in 0.1% formic acid)(25+75,V/V).The flow rate was 1.0 mL/min;the detection wavelength was 200 nm.Results The standard curve of glucosamine hydrochloride was linear over the range in 0.2~26.0 μg(r=0.999 982).The...

Abstract:Objective A high performance liquid chromatography(HPLC) coupled with hydride generation and atomic fluorescence spectrometry is proposed for the determination of methyl mercury in seafood.Methods Samples were extracted by 25% potassium hydroxide and the methylmercury-thiol complex was separated on a Supelco C18(150 mm×4.6 mm,5 μm) reverse phase column and online UV-digested.After reduced to element Hg by KBH4,the element Hg was selectively detected by AFS in an Ar/H2 flame under optimized condi...

Abstract:Objective To establish a method for detecting perfluorooctanesulfonate(PFOS) and perfluorooctanoic acid(PFOA) in three kinds of animal food.Methods Samples were extracted by methanol and cleaned-up by SPE in a WAX column.The analytes were separated and detected on ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) by C18 column with mobile phase of methanol and 2 mmol/L ammonium acetate aqueous solution,and performed with negative EST at multiple reaction monitoring(MRM...

Abstract:Objective A sensitive liquid chromatography-tandem mass spectrometry(LC-MS/MS) method was developed for the simultaneous determination of 3 kinds of banned nitroimidazole veterinary drug and their metabolites including metronidazole,ronidazole,dimetridazole,metronidazole-OH(the metabolite of metronidazole),and 2-hydroxymethyl-1-methyl-5-nitroimidazole(the metabolite of ronidazole and dimetridazole) in milk and milk powder.Methods The protein in samples were precipitated with acetonitrile and the...

Abstract:Objective Considering the disadvantages of complicated operation steps for spectrophotometry methods with plenty of influencing factors,and the high cost for ICP-MS,an applicable determination method for aluminium in flour foods was established by microwave digestion and GFAAS assay.Methods After digested in microwave,the sample was introduced into GFAAS for analysis.The absorption resonance line at 257.4 nm of samples and standard series was compared and the content of aluminium was measured qu...

Abstract:Objective To detect the carriage of Salmonella in raw meat for the prevention of Salmonella food poisoning.Methods Dot-ELISA was used to detect the contamination of Salmonella from 85 pork,71 beef and 101 mutton raw meat samples,and compared with the routine isolation and culture identification technique in some slaughter houses in Xining.Results The positive rate was 76.47%(65/85) for pork,55.44%(56/101) for beef and 46.48%(33/71) for mutton detected by Dot-ELISA;and the positive rate was 78.82...

Abstract:Objective Food tracing means collecting information through the food chain from production to consumption,either backward or forward,helping to identify the source and distribution of suspected foods in foodborne disease outbreaks.An outbreak caused by Enterohemorrhagic Escherichia.Coli O104:H4 infection has happened during May to July in 2011 in Germany.With the help of food tracing investigation system,the source,fenugreek seeds imported from Egypt,was eventually identified.This article gives ...

Abstract:Objective To analyze the epidemiological characteristics of foodborne disease outbreaks in China in 2006—2010.Method The data of foodborne disease outbreaks during 2006 to 2010 collected by the Emergency Public Health Surveillance Network of the Ministry of Health of the People’s Republic of China was analyzed.Results A total of 2 023 outbreaks of foodborne diseases,which caused 62 920 ill and 967 death cases,were reported in China.The etiologies of outbreaks were identified by epidemiology.The ...

Abstract:Objective To monitor and assess the residues of malachite green(MG)and its metabolites leucomalachite green(LMG) in freshwater fish.Methods Twenty eight kinds of 207 fish samples were collected from retailers in six cities of Fujian Province.After samples being homogenized and pre-treated,the concentration of total MG in samples was quantified by high performance liquid chromatography(HPLC) coupled with fluorescence detector.The positive samples analyzed by HPLC were confirmed by HPLC-mass spect...

Abstract:Objective To investigate the chemical contaminants and harmful factors in some food products in Jincheng.Methods Metal elements and additives in food were examined according to GB/T 5009—2003 "food sanitation methods".Pesticide residue were detected according to NY/T 761—2008 "the People’s Republic of China of agricultural industry standards".Results In 219 grain,egg product,meat product,vegetable,fungus and fish samples,the lead content in 2 samples and cadmium content in 3 samples were over th...

Abstract:According to The Law of Food Safety,the related requirements of the State Council and the Ministry of Public Health,and the program of systemic revision of national food safety standards,the Hygienic Standards for the Use of Food Additives(GB 2760—2007) was revised by the National Institute of Nutrition and Food safety of China CDC in 2011.The revised parts are mainly on the regulations of using food additives,food processing aids and flavorings.The revision of the National food safety standard-...

Abstract:Oral tolerance refers to a state of active inhibition of immune response to a food antigen which has been exposed previously via oral route.With exposure to a large extent of food antigens every day,adverse immunologic reactions to an antigen only take place in a small proportion of individuals because of the existence of oral tolerance.Food allergy is one of hot topics in related fields.Establishing an animal model is a direct way to explore food allergy.Avoiding oral tolerance of an animal to ..

Abstract:Pathogenic microorganism in aquatic products is one of the most important factors in human food-borne diseases.Several new sterilization technologies such as high pressure sterilization,irradiation sterilization,electrolyzed water sterilization and bacteriostatic agents are provided in this article.Their basic principle,the recent development in application and the future trend of these techniques used in seafood industries are also briefly discussed. 更多还原

Abstract:With an increasing usage of genetically modified(GM) foods for human consumption,there is a growing need for the development of approaches suitable for assessing the potential allergenicity of GM foods.The progress of current assessing approaches for the allergenic potential of GM foods,including decision tree,sequence homology,serology screening,digestive stability(pepsin resistance) and animal models were discussed,and an overview on the present condition and recommendations for its developmen...