Abstract:Objective To analyze the epidemiological characteristics of infectious diarrhea in China and provide theoretical support to control the infectious diarrhea.Methods Using a descriptive epidemiologic statistics to analyze the infectious diarrhea cases collected from China Information System for Diseases Control and Prevention.Results There were 746 551 cases of infectious diarrhea reported in 2010 in China with an annual morbidity of 55.93/105,which was higher than that reported in 2009.And 53.57%...

Abstract:Objective To understand the situation of pollutants in agricultural products from two regions in Zhenjiang with different cancer incidence,so as to provide evidence for reducing risk factors of cancer.Methods Lead,cadmium,mercury,aflatoxin and pesticides in agricultural food products were examined.Results Lead,cadmium,mercury were detected in 38,44 and 21 of 91 agricultural samples,respectively.There was no difference on the detection rate of metal pollutants between two regions.The amount of le...

Abstract:Objective To develop a specific PCR detection method for L.monocytogenes and to investigate the contamination of L.monocytogenes in different foods in Chengdu city.Methods A total of 135 random samples from sorts of meat,fish,dairy,vegetable and other ready-to-eat food were collected from free markets and supermarkets.The samples were followed an enrichment period with LB1 and LB2 broth,then separated and identified by using PALCAM medium,TSA-YE agar and CHROMagarTM Listeria.The virulence gene i...

Abstract:Objective To develop a rapid chemiluminescence enzyme immunoassay(CLEIA) for detecting enrofloxacin(ENR) in milk.Methods Anti-ENR antibody was passively adsorbed onto the walls of polypropylene plates.ENR conjugated with labeled alkaline phosphatase(ALP) was used to compete with the ENR in milk for the limited specific capability of ENR antibody on plates by solid-phase antibody binding reaction.Through separating unbounded labeled antigen and determining of chemiluminescence,the content of the ...

Abstract:Objective To provide an animal model for the evaluation of memory improvement assisted by functional foods.Methods The behavior performance of mice after sleep interruption(SI) by a rotating drum for 5,10 and 15 days were inspected by Open Field test(OF),Morris Water Maze test(MWM) and Dark Avoidance test(DA).Results The activities and cognitive ability of mice were not different between 5d SI group and 5d control group.Comparing the 10d SI group with 10d control group,the total distance,movemen...

Abstract:Objective To study the molecular epidemiology of two Salmonella enteritidis strains in two food-poisoning accidents occurred at the same time but separated in a distance of 210 kilometers away from each other.Methods Eight S.enteritidis stains isolated from patients and food residues in two food-poisoning accidents were identified,and single colonies were picked out to multiply on agar plates.The total DNA of isolates was digested with restriction enzyme Xba I,and run pulsed-field gel electropho...

Abstract:Objective To determine 12 inorganic elements in Vitis amurensis by atomic absorption spectrometry(AAS).Methods Samples were dried and crushed with a mechanical device.Weighing 1.000 0 g of crushed Vitis amurensis and putting into a 150 ml Erlenmeyer flask.Adding 20 ml of acid digestion reagent(HNO3 ∶HClO4=4∶1 by volume)and then heated on an electric hot plate.The contents of K,Ca,Na,Mg,Fe,Mn,Zn,Cu and Cr in Vitis amurensis were determined by flame atomic absorption spectrometry,the contents of C...

Abstract:Objective To investigate the effect of bleaching agent hydrogen peroxide and alkali on anthocyanidin in pistachio and the necessity of bleaching techniques.Methods High performance liquid chromatography(HPLC) with diode array detector(DAD) was used to determinate the content of cyanidin-3-O-glucoside and cyanidin-3-O-galactoside in pistachio.The appearances of hard shell,kernel skin and kernel of raw,natural and bleached pistachio were compared.Results Anthocyanidins were rather sensitive to hyd...

Abstract:Objective To acquire the optimum preservatives compounding to inhibit Bacillus thuringiensis.Methods The inhibitive effect of preservatives on Bacillus thuringiensis was examined by susceptibility test paper and minimum inhibitory concentration(MIC) methods;the optimized combination of preservatives was screened by orthogonal design,and the inhibitive effect of the best combination of preservatives was also checked.Results The results showed that Bacillus thuringiensis were not sensitive to sodi...

Abstract:Objective To establish a solid phase extraction(SPE) with atom absorption spectrometry(AAS) for the determination of lead in soy sauce samples.Methods The samples were pretreated by microwave digestion.After the digested solution being adjusted to pH 5.5 by ammonium acetate,the solution was then passed through a DigiSEP-Blue cartridge pretreated by 5 ml of 1 mol/L ammonium acetate for the removal of large amount of salt.The eluate from the DigiSEP-Blue cartridge washed by 8ml of 2 mol/L nitric a...

Abstract:Objective To establish a method based on solid-phase extraction and gas chromatography-mass spectrometry(GC/MS) for the determination of 3-chloro-1,2-propanediol(3-MCPD),1,3-dichloro-2-propanol(1,3-DCP) and 2,3-dichloro-1-propanol(2,3-DCP) in soy sauce.Methods Soy sauce samples were spiked with deuterium-labeled isotope internal standards and loaded on market-available SPE cartridges,then the analytes were extracted with ethyl acetate and derivatized with N-heptafluorobutyrylimidazole(HFBI).The ...

Abstract:Objective To establish an ultraperformance liquid chromatography with electrospray tandem mass spectrometry(UPLC-MS/MS) method for the determination of cholesterol in hotpot oil,hogwash oil and waste oil.Methods Oil samples were separated on a Waters ACQUITY UPLC BEH C18 column(2.1 mm × 50 mm,1.7 μm) with a mobile phase of methanol + formic acid(999+1).Cholesterol and internal standard(2,2,3,4,4,6-d6 cholesterol) were detected by multi-reactions monitoring(MRM) technology on the transitions of ...

Abstract:Objective To establish a method of determining vitamin B12 in health food products by liquid chromatography electrospray ionization tandem mass spectrometry(LC-MS/MS).Methods After mixing samples with internal standard solution of ginsenoside Re,the mixtures of samples were extracted by solid phase extraction,and separated on a Bio Basic-18 PIONEER column(150 mm×2.1 mm,5 μm) by a mobile phase of methanol(A) + water(B) with the gradient elution program.Detection was carried out by a liquid chroma...

Abstract:Objective To detect sodium cyclamate in food by gas chromatography method.Methods Sodium cyclamate was reacted with nitrite in sulfuric acid medium,the generated cyclohexanol nitrate was separated by large-diameter capillary column DM-FFAP(30m×0.53 mm×0.5 μm),and detected by GC-FID.Results The linear range was 0.010-2.0 mg/ml(r= 0.999 9),the relative standard deviation was 3.2%-6.1%,and the recovery was 90.0%-105.2%.Conclusion The method is simple,rapid,accurate and more reliable for the determi...

Abstract:Objective To test ractopamine with the method of time-resolved fluoroimmunoassay(TRFIA) for the determination of.Methods RAC-BSA was coated by physical adsorption onto a 96 well microtitre plate,which was then competed with RAC in the sample for limited quantity of anti-RAC antibody labeled by Eu3+.Ractopamine in urine could be determined by direct competition method.Results The assay sensitivity of the method was 0.01ng/ml.The correlation coefficient of dose-response curve was 0.998 5.The recov...

Abstract:Objective To establish a new colorimetric assay method for the determination of total ginsenoside in Ginseng health foods.Methods Samples were purified by macroporous adsorption resin,the reaction of anthrone with glycon in ginsenosides was assayed by colorimetric method.Ginsenoside Re was used as a reference material.Results There was a good linearity in the range of 5-200 μg/ml of ginsenoside,the regression equation was y=0.369+0.994x,the correlation coefficient r=0.999 5.The recovery rate was...

Abstract:To analyze the problems on the treatment of current food safety accidents;mainly focused on the organization and coordination,information reporting,identifying the nature of accident,identifying the number of cases and the capability of local centers for disease control and prevention after the accident being occurred.Based on the existed problems,the treatment and disposition system and security mechanism for food safety incidents were discussed.

Abstract:The similarities and differences of published Food Allergen Labeling Act of our main trading partner countries were analyzed and summarized,including the coverage of measures,list of allergens and exemption list,ways of labeling,and requirements for labeling cross-contaminants.Finally,China’s response measures to these regulations or standards were also put forward.

Abstract:Objective Through the analysis on a case of food poisoning punishment in catering services,the applicability and operability of the punishment part in the Food Safety Law of the People’s Republic of China were discussed.Methods The limits of penalty in a case of food poisoning was analyzed and discussed.Results There were some defects and weakness on the applicability and operability of executing penalty in the Food Safety Law of the People’s Republic of China.Conclusion The law and its supporti...

Abstract:The feasibility and the necessity of implementing nutrition labeling for Chinese dishes were discussed.Suggestions for the rules of nutrition labeling on dishes for food and beverage industries(draft) were provided,such as marking the calculation of nutrients in Chinese dishes on nutrition labels,the nutrient composition data should be certificated,standardizing the ingredients of dishes,establishing nutrition barcode to unify various food nutrition labels,standardizing dimension units for marki...

Abstract:Objective To survey the change of nutrition labeling on prepackaged food in Hangzhou supermarkets before and two years after promulgating the Regulation for Food Nutrition Labeling.Methods Nutrition facts,nutrition claims and nutrient function claims on prepackaged food in a supermarket were copied with prepared questionnaire in 2008 and 2010,and a normative judgment was made.Results There were 4 693 and 5 526 kinds of domestic prepackaged food investigated in 2008 and 2010,respectively.The numb...

Abstract:Objective To provide scientific evidence of diagnosing food poisoning caused by Vibrio parahaemolyticus(VP) to reduce the infection risks in Guangzhou.Methods A retrospective analysis of epidemiology characteristics was conducted on analyzing the Case Report Forms and Individual Registration Forms reported from food poisoning accidents in the years 1997—2007 in Guangzhou.Results The incidence rate of food poisoning caused by VP was increasing yearly.There were 97 food poisoning outbreaks caused ...

Abstract:Objective To investigate and characterize the contamination of seafoods caused by Vibrio parahaemolyticus in Shanghai markets.Methods Based on the method of GB/T 4789.7—2008 Microbiological Examination of Food Hygiene: Examination of V.parahaemolyticus,the contamination of seafoods caused by V.parahaemolyticus from different pre-determined monitoring sites in Shanghai was qualitatively and quantitatively determined.Results A total of 941 seafood samples from wholesale markets,farmers’ markets,su...

Abstract:As indicator organisms,standards for aerobic plate count,coliforms,Escherichia coli,enterobacteriaceae applied in each country were different.Based on the comparison and analysis of microbiological criteria of foods in Australia,New Zealand,European Communities,Canada and Hong Kong,recommendations and technical basis for the elaboration of general microbiological criteria of China’s national food safety standards were provided. 更多还原

Abstract:The article reviewed the basic principle and main influencing factors of immunomagnetic beads separation techniques(IMBS) and its application in microbiology.An emphasis was put on introducing the conjunction of IMBS with other methods,such as standard culture methods,polymerase chain reaction and immunoassays,to make the detection of pathogenic organisms more effective,to increase the detection rate and reduce turaround time.IMBS techniques provided a new technical strategy for diagnostic micro...