Simultaneous determination of 15 kinds of organophosphate esters and carbamate pesticide residues in tea drinks by ultra-high-performance liquid chromatography tandem mass spectrometry
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1.Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Prevention and Control, Beijing 100013, China;2.Yanjing Medical College, Capital Medical University, Beijing 101300, China;3.Department of Medical Science and Technology, Guiyang Healthcare Vocational University, Guizhou Guiyang 550081, China

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R155

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    Abstract:

    Objective A QuEChERS ultra-high-performance liquid chromatography tandem mass spectrometry analytical method for the determination of 10 kinds of organophosphate esters pesticides and 5 kinds of carbamate pesticides in tea drinks was established.Methods The extraction solution was purified with 150 mg MgSO4, 25 mg Primary-secondary comine and 2.5 mg graphitized carbon black. After centrifugation at 14 000 r/min at 4℃ for 10 min and the supernatant was transferred, diluted with an equal volume of ultra-pure water. The pesticides were separated on a Waters ACQUITY BEH C18 (2.1 mm×100 mm, 1.7 μm) chromatographic column with gradient elution using 0.1% formate-acetonitrile and 0.1% formic acid aqueous solution. Pesticides were detected via multiple reaction monitoring mode.Results The method showed a good linear range of 5-160 μg/L (R2>0.99). The limits of detection and limits of quantification were 0.05-5 μg/L and 0.15-15 μg/L, respectively. Mean recoveries of 15 kinds of pesticides were 71.2%-103.2%. Intraday (n=6) and interday (n=3) relative standard deviations were 0.1%-13.8% and 0.4%-12.2%, respectively. Matrix effects were 84.1%-114.3%, and could be considered to have no matrix effect. Acephate was detected in 2 out of 43 commercial tea drinks, it suggests that pesticide residues in tea drinks may cause increased health risks for people.Conclusion The method was simple to operate, rapid, accurate, and suitable to detect fifteen kinds of pesticides in different tea drinks.

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YAO Kai, GAO Zihan, ZHANG Jing, QI Yan, ZHONG Nannan, SHAO Bing. Simultaneous determination of 15 kinds of organophosphate esters and carbamate pesticide residues in tea drinks by ultra-high-performance liquid chromatography tandem mass spectrometry[J].中国食品卫生杂志,2024,36(12):1349-1358.

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  • Received:May 30,2024
  • Online: March 19,2025
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