Rapid determination of seven pyrethroid pesticides in vegetables by combining QuEChERS with disposable polyethylene pipet assisted dispersed liquid-liquid micro extraction
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1.School of Public Health, Wuhan University of Science and Technology; Hubei Provincial Key Laboratory of Occupational Hazard Identification and Control, Hubei Wuhan 430065, China;2.Center For Disease Control of Yiling District, Hubei Province, Hubei Yichang, 443100, China

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R155

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    Abstract:

    Objective A new method for the separation and enrichment of pyrethroid pesticides in vegetables was developed by combining QuEChERS with disposable polyethylene pipet assisted dispersed liquid-liquid micro-extraction (DPP-DLLME).Methods Firstly, acetonitrile was used as extraction agent for separation of pyrethroid pesticides from vegetable samples, and the collected acetonitrile extract was purified, then used as the dispersant of DPP-DLLME. A disposable dropper was used as the extraction vessel for DPP-DLLME, and n-octanol was used as the extractant. After phase separation, the extractant was floating on the aqueous solution and concentrated in the narrow neck of the pipet, which can be directly withdrawn by a microsyringe for instrument analysis.Results Under the optimized extraction conditions, the linearity range was 0.5-200 ng/g with correlation coefficient r2 higher than 0.995 for seven pyrethroid pesticides. The limits of detection were in the range of 0.04-0.1 ng/g, and the limits of quantitation were in the range of 0.1-0.5 ng/g. The recoveries at three spiking levels of 1, 10 and 100 ng/g were in the range of 94.5%-110.5% with the RSDs less than 9.0% (intra-day) and 8.2% (inter-day).Conclusion The method was simple and effective, which provided a new choice for the detection of pyrethroid pesticides in vegetables.

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YANG Jinjie, YUE Shiwen, QIN Sheng, ZHAO Qin. Rapid determination of seven pyrethroid pesticides in vegetables by combining QuEChERS with disposable polyethylene pipet assisted dispersed liquid-liquid micro extraction[J].中国食品卫生杂志,2024,36(10):1130-1137.

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  • Received:May 30,2024
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  • Online: February 07,2025
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