Cronobacter spp. are foodborne opportunistic pathogens known for their significant environmental adaptability and wide-ranging distribution. These organisms have the potential to cause neonatal infections through the contamination of infant powdered formula， resulting in serious diseases， such as necrotizing enterocolitis， sepsis or meningitis， thereby posing a serious threat to the health and life of newborns and premature infants. The recent rapid progress in high-throughput sequencing technology and bacterial genomics has greatly promoted research in areas such as the genomic analysis of Cronobacter， molecular evolution and mechanisms of variation， virulence genes， identification genes， and drug resistance gene mining. These advancements have significant scientific implications for the prevention and treatment of Cronobacter infections. This review aims to offer a thorough analysis of the present status and forthcoming directions in Cronobacter genomics research， providing essential theoretical groundwork for the development of novel approaches in identification， prevention， and treatment of Cronobacter infections.