Objective Laboratory analyses were performed for a case of suspected botulinum poisoning incident that occurred in November 2023，and to analyze the whole genome of the strain of C.botulinum type A isolated.Methods Real time fluorescence quantitative polymerase chain reaction is applied to rapidly detect botulinum toxin genes in food， case feces and environmental samples. According to GB 4789.12—2016，the samples were subjected to pre-treatment.After being inoculated with blister meat culture medium and TPGYT culture medium for 5 d of enrichment， toxin detection，confirmation，and qualitative experiments were conducted through animal experiments.The strains were isolated and purified using egg yolk agar plates，and identified using API 20A and VITEK MS.Simultaneously，WGS was performed on the isolated strains to analyze their genetic characteristics.Results The fecal sample of the case was positive for type A botulinum toxin gene， and type A botulinum toxin was detected through animal experiments. C.botulinum type A RD1 and RD2 was successfully isolated using egg yolk agar plates，all other samples were negative.Whole genome sequencing analysis showed that the bacterium is C.botulinum，with a total of 12 toxin coding related genes and 3 phage sequences on the chromosome.Wg-SNPs analysis showed that RD1 and RD2 has the closest genetic relationship with BrDura.Conclusion This experiment was identified according to national standards and the fluorescence quantitative PCR method established in our laboratory. The detection plan and results provide reference for clinical type A botulism.