Objective To provide experience for the response to Staphylococcus aureus enterotoxin （SEs） poisoning outbreak， lab test and whole genome sequencing （WGS） technology was used to analyze a SEs poisoning outbreak.Methods Suspected contaminated food， anal swabs and environmental smear samples in this event was collected. enzyme-linked immunosorbent assay （ELISA） for five SEs （SEA， SEB， SEC， SED， SEE）， and real time PCR for genes of SEs （sea， seb， sec， sed， see） were performed. Drug resistance phenotype and WGS were performed in Staphylococcus aureus strains isolated from samples， and a cgSNP evolutionary tree was constructed.Results SEs were negative for three suspected contaminated foods. Real time PCR for enrichment solution of the three suspected contaminated foods were all nuc+/seb+， and 9 isolates of seb+ S. aureus were isolated from three suspicious contaminated foods， among which two isolates of type t78 and one isolate of type t437 from food 1， and the isolates from food 2 and food 3 were all type t437， with the same spa typing isolates having the same drug-resistant phenotype. Two isolates of S. aureus were isolated from one environmental smear， which negative for five SEs genes and presenting the same type t571. Three independent and genetically distant branches were formed in a cgSNP evolutionary tree constructed based on nine food isolates and two environmental isolates of S. aureus. Nine food isolates were positive for SEB by ELISA.Conclusion This incident is a suspected SEB intoxication， and whole genome sequencing may provide more comprehensive bioinformatic data to respond to such incidents.