Objective The concentration of enniatins （ENNs） in wheat flour from Gansu Province in 2022 was investigated.Methods Totally 80 wheat flour samples were collected from 15 cities in the Gansu Province. The samples were statistic with national handbook for monitoring food contamination and hazardous factors. The data were analyzed by correlation analysis.Results The detection rates of four toxins in wheat flour sold in Gansu Province were ENNB13.75% （11/80，13.75%），ENNB1 （9/80，11.25%）， ENNA and ENNA1（6/80，7.50%）. Non of ENNs was detected in the wheat flour samples from Wuwei， Baiyin and Dingxi in Gansu Province， and at least one ENNs was detected in the other 12 regions.Conclusion Four kinds of ENNs were detected in wheat flour in Gansu Province， but the contents were lower than that reported all of the world， which proved that the pollution degree of ENNs in wheat flour sold in Gansu Province was low.