Objective To develop a rapid and quantitative method for the detection of enrofloxacin in chicken and pork using magnetic solid phase extraction （MSPE） combined with surface enhanced Raman spectroscopy （SERS）.Methods Magnetic Fe₃O₄@ covalent organic framework （COF） nanocomposites were synthesized and applied as absorbent to separate and enrich enrofloxacin. The SERS spectra of enrofloxacin were collected by a portable Raman spectrometer with Ag nanoparticles as enhancing substrate. The characteristic SERS signals of enrofloxacin were used to realize its quantification.Results Intensities of SERS signals at 745.77 cm^-1 and the logarithmic values of the concentrations of enrofloxacin in the range of 5.0×10^-7 to 1.0×10^-5 mol/L displayed a good linear relationship with a coefficient of determination of 0.962. The limit of detection and limit of quantitation were 0.07 and 0.23 μg/g respectively. The recoveries of enrofloxacin in chicken and pork were 80.97%~100.98% with relative standard deviations of 1.6%~4.6%.Conclusion The developed method has the advantages of simple in operate， short in time， high in sensitivity and stability， which provides a new method for the rapid detection and on-site detection of enrofloxacin.