Home > Archive>Volume 36, Issue 4, 2024 >389-393. DOI:10.13590/j.cjfh.2024.04.004
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Detection of the stool and anal swab samples from outbreaks caused by Campylobacter by real-time PCR
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1.Tongzhou District Center for Disease Prevention and Control, Beijing 101100, China;2.Shunyi District Center for Disease Prevention and Control, Beijing 101300, China

Clc Number:

R155

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    Abstract:

    Objective To lay the foundation of quickly and effectively response to such epidemics for laboratories， the detection of the real-time PCR and culture test results of stool and anal swab samples from 3 outbreaks caused by Campylobacter was compared.Methods Biological samples from the 3 outbreaks of Campylobacter were collected. Bacterial culture was performed on the samples using filter culture method. DNA was extracted from the original samples， 24 h enriched samples and 48 h enriched samples. Kappa test was used to analyze the results of real-time PCR and culture method.Results The real-time PCR detection sensitivity of original samples， 24 h enriched samples and 48 h enriched samples were 90.91%， 97.22% and 100%. And the detection specificity were 75.00%， 84.00%和78.95%， respectively. Kappa value for the consistency analysis in culture method with real-time PCR based on original samples， 24 h enriched culture samples and 48 h enriched samples were 0.643， 0.813 and 0.785， respectively.Conclusion The combination of real-time PCR detection and culture detection is an effective laboratory testing method for the respond of Campylobacter outbreaks.

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ZOU Lin, JIA Nan, ZHANG Ping, ZHEN Bojun, GUO Xiaochen, WANG Fang, JI Guoqiang, YAN Aixia, KANG Ying, MA Hongmei, LI Ying. Detection of the stool and anal swab samples from outbreaks caused by Campylobacter by real-time PCR[J].中国食品卫生杂志,2024,36(4):389-393.

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  • Received:March 24,2023
  • Online: July 18,2024
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