Objective To provide reference for the disposition and clinical treatment of poisoning incidents， rapid toxin screening and the clinical symptoms and treatment of a case of Gymnopilus dilepis poisoning were conducted and discussed.Methods Ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry was used to detect the remaining poisonous mushroom in the contaminated food. Based on an epidemiological investigation， case data were collected. The results of the epidemiological investigation and the liver and kidney indexes of the patients were analyzed.Results Psilocybin and muscarine were detected in the Gymnopilus dilepis consumed by the patients. The symptoms were similar to those of psilocybin and muscarine poisoning， which included hallucinations， nausea， vomiting， abdominal pain， and diarrhea. The liver and kidney functions were normal.Conclusion The poisoning event was caused by psilocybin and muscarine in Gymnopilus dilepis.