Molecular tracing of a suspected foodborne disease event caused by Bacillus cereus
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1.Shanghai Municipal Center for Disease Control & Prevention, Shanghai 200336, China;2.Changning District Center for Disease Control & Prevention, Shanghai 200051, China;3.Minhang District Center for Disease Control & Prevention, Shanghai 201101, China

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R155

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    Abstract:

    Objective To trace Bacillus cereusB. cereus) from foodborne disease outbreaks toidentify pathogens and cut off transmission.Methods Pulsed-field gel electrophoresis (PFGE) was performed. Furthermore, 12 isolates of B. cereus were subjected to PFGE. Subsequently, whole-genome sequencing (WGS) analysis was conducted on ten of these isolates. The WGS data were analyzed and assembled using BioNumerics software. Multilocus sequence typing (MLST), virulence gene profiles, and single nucleotide polymorphisms (SNPs) were analyzed using assembled sequences.Results PFGE analysis classified the 12 B. cereus strains into nine pulsotypes. The three B. cereus isolates with the same PFGE pattern belonged to ST1435, and there were only three SNPs in the three ST1435 strains. The two B. cereus isolates with the same PFGE patterns were ST24 with one SNP between them, and the two ST24 isolates harbored hlbACD. These results indicate that the B. cereus isolates belonged to the same clone. The remaining three B. cereus strains also contained hlbACD.Conclusion Food-borne illness events caused by B. cereus are complex and are sources of contamination. Therefore, it will be necessary to strengthen the hygiene surveillance of food sources and workers and to pay more attention to cleaning and disinfecting environments and facilities, which will be important for preventing and controlling foodborne diseases.

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CHEN Wenjie, LU Donglei, HUANG Zheng, GU Qifang, BI Lei, FANG Zhixin, YU Ying, WANG Jing, XIAO Quan, ZHANG Hongzhi. Molecular tracing of a suspected foodborne disease event caused by Bacillus cereus[J].中国食品卫生杂志,2023,35(12):1799-1804.

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History
  • Received:August 19,2022
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  • Online: April 03,2024
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