Application of colloidal gold immunochromatography on rapid detection of quinolones in bean sprouts
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1.Sichuan Institute of Food Inspection, Sichuan Chengdu 610000, China;2.Key Laboratory of Baijiu Supervising Technology for State Market Regulation, Sichuan Chengdu 610000, China

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R155

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    Abstract:

    Objective To study the applicability for determination of norfloxacin, ofloxacin, difloxacin, enrofloxacin, ciprofloxacin, fleroxacin, dafloxacin, pefloxacin, lomefloxacin and salafloxacin in bean sprouts by colloidal gold immunochromatography.Methods Took representative bean sprout ground 2 g, extracted with 5 mL extraction solution, and then filtered and diluted 2 times. Drew the diluent into the gold standard micropore, incubated at room temperature, and inserted the test strip into the gold standard micropore. The reaction results were interpreted by comparing the color depth of the detection line (T line) and the control line (C line). The experiment also studied the cross-reaction of 6-benzyladenine, 4-chlorophenoxyacetic acid, metronidazole, urea and clopidourea, and the reliability of the method was investigated by real positive samples and multi-level spiked samples.Results The detection limit of the method could reach 5 μg/kg ; no cross reactions occurred with 6-benzyladenine, 4-chlorophenoxyacetic acid, metronidazole, urea and clopidourea under the condition of routine detection level; The results of real positive samples and multi-level spiked samples confirmed that the method was reliable.Conclusion The method is sensitive, accurate and fast, which is suitable for the field rapid detection of quinolones in bean sprouts.

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LIU Mei, YAO Huan, YU Xiaoqin, Xia Mengting. Application of colloidal gold immunochromatography on rapid detection of quinolones in bean sprouts[J].中国食品卫生杂志,2023,35(12):1722-1726.

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History
  • Received:June 20,2022
  • Revised:
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  • Online: April 03,2024
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