Objective To establish a method for the determination of chloramphenicol drugs in hen eggs by ultra-performance liquid chromatography-tandem mass spectrometry with Captiva EMR-Lipid pretreatment.Methods Samples were extracted with ammoniated acetonitrile，then purified with Captiva EMR-Lipid，and finally detected by UPLC-MS/MS with electrospray ionization in multiple reaction monitoring mode via positive and negative ions scanning. Compounds were quantified by internal standard method.Results There were good linear relationships for 3 compounds in the range of 0.03-6.0 μg/L with correlation coefficients （r） not less than 0.995. The limits of detection of chloramphenicol （CAP）， florfenicol （FF） and florfenicol amine （FFA） were 0.02， 0.01， 0.04 μg/kg， respectively. And the limits of quantitation were 0.06， 0.03， 0.12 μg/kg， respectively. The method recoveries were between 92.3%-109.6%，with relative standard deviations（n=6） between 1.4%-8.2%.Conclusion With simple operation and high accuracy， the method was suitable for the determination of CAP， FF and FFA in hen eggs.